安徽农业科学2016，Vol.44Issue(14)：149-151,3.
福建地区2株猪嵴病毒 VP1基因的克隆及遗传进化分析
Cloning of Porcine Kobuvirus VP1 in Fujian province and Phylogenetic analysis
摘要
Abstract
Objective] The current situation of the epidemic and genetic variation of the porcine Kobuvirus (PKV) in diarrhea pigs in Fujian province were investigated.[Method] The special primer was designed according to the sequence of PKV deposited in GenBank.The VP1 gene of PKV in intestinal samples collected from pig -raising farm was amplified with RT -PCR method, and then, the amplified target frag-ment was cloned and sequenced.The sequences of two PKV strains were analyzed and compared by means of bioinformatics software.[Re-sults] The results showed that there was high nucleotide homology between VP1 gene of CH /FJNP/12L /2015and K -30 -HU /2008 /HUN, which was 88.1%; amino acid homology, 95.3%.The homology in the nucleotide and amino acid of CH /FJNP/12W1 /2015 with 714441 /CAOLANH -VH /2012 -2 -21 was 88.2% and 96.1%, respectively.The two isolates had no obvious homologus recombination occurred with other strains.[Conclusion] speculated that the frequent international livestock trade, and is now a modern and convenient means of trans-port, in convenience to our lives, but also accelerate the spread of swine crest virus strains.[Conclusion] It is speculated that it resulted from the frequent international trade of livestock and poultry, and convenient modern transportation tools, which also accelerated the transmission of PKV.关键词
猪嵴病毒/VP1 基因/同源性Key words
Porcine kobuviruses/VP1 gene/Homology分类
畜牧业引用本文复制引用
张永军,胡永浩..福建地区2株猪嵴病毒 VP1基因的克隆及遗传进化分析[J].安徽农业科学,2016,44(14):149-151,3.基金项目
中国农业产业体系（CARS －39）。 （）
