安徽农业科学2016，Vol.44Issue(14)：152-154,161,4.
双分子荧光互补技术（BiFC）分析玉米 MAPK5与 bZIP72蛋白的相互作用
Interaction Between MAPK5 and bZIP72 of Maize Using Bimolecular Fluorescence Complementation (BiFC) Assay
曹建美1
作者信息
- 1. 中国科学院上海辰山植物研究中心，上海辰山植物园，上海松江201602
- 折叠
摘要
Abstract
Objective] To analyze the interaction between MAPK5 and bZIP72.[Method] Two BiFc plasmids (pN-MAPK5 and pC-bZIP72) were constructed, then transfected into onion epidermal cells via gene-gun approach.The interaction between MAPK5 and bZIP72 was examined by confocal laser scanning microscopy.[Result] Yellow fluorescence was observed in the onion epidermal cells transfected with ZmMAPK5 and ZmbZIP72.[Conclusion] This research indicates that ZmMAPK5 and ZmbZIP72 interacts with each other in vivo.关键词
ZmMAPK5/ZmbZIP72/双分子荧光互补(BiFC)/蛋白互作Key words
ZmMAPK5/ZmbZIP72/Bimolecular fluorescence complementation (BiFC)/Protein interaction分类
农业科学引用本文复制引用
曹建美..双分子荧光互补技术（BiFC）分析玉米 MAPK5与 bZIP72蛋白的相互作用[J].安徽农业科学,2016,44(14):152-154,161,4.
