安徽农业科学2016，Vol.44Issue(14)：169-171,3.
黔产景天三七干・鲜品中没食子酸含量比较研究
Comparative Study on the Content of Gallic Acid in the Fresh and Dried Herb of Guizhou Sedum aizoon L
摘要
Abstract
[Objective] The determination method of the content of gallic acid in Guizhou Sedum aizoon L.was established and the method forthe comparative analysis of its content in the fresh and dried raw material from different harvest time and different parts was conducted.[Method]HPLC method was adopted and the separation was carried with the column of Phenomex Gimini C18 (250 mm ×4.6 mm, 5 μm), the mobilephase of methanol -0.05% phosphoric(5∶95) at the flow rate of 1.0 mL/min, the detection wavelength of 273 nm and column temperature of 25℃.[Results] The linear relation(r =0.999 9) of gallic acid was good in the range of 0.018 -0.570 μg.The average recovery rate was 100.44%, the value of RSD was 1.63%(n =6).[Conclusion] The method is rapid, simple and accurate, and can be used for the quality control ofthe fresh and dried medicinal material of Guizhou Sedum aizoon L.from different parts and harvest seasons.关键词
景天三七/高效液相色谱法/没食子酸/含量分类
农业科技引用本文复制引用
何晶晶,杜江..黔产景天三七干・鲜品中没食子酸含量比较研究[J].安徽农业科学,2016,44(14):169-171,3.基金项目
国家自然科学基金项目（81450657）。 （）
