安徽医科大学学报2016，Vol.51Issue(6)：773-777,5.
人参对胃癌 SGC-7901侧群细胞裸鼠移植瘤生长及 Bcl-2、Bax表达的影响
Effect of Ginseng on human gastric cancer SGC-7901 side population cells xenograft tumor in nude mice and the expression of Bcl-2,Bax
摘要
Abstract
Objective To explore the affection of Ginseng on the growth of humangastric cancer SGC-7901 side population(SP) cells xenografts in nude mice and expression of Bcl-2,Bax.Methods Sorting out SP cells and non-side population(NSP) cells by flow cytometry;preparation of Ginseng decoction;tumor volume were measured to draw tumor growth curves and the tumor weight inhibition rates were calculated with tumor weight .The expres-sions of Bcl-2,Bax were detected by immunohistochemical technology , preparation containing Ginseng drug serum , Western blot technique was used to detect the expression of Bcl-2, Bax.Results The volume of transplanted tumors in SP cells NS group was bigger than group of NSP cells NS group ( P<0.05 ) .The tumor volume of NS was bigger than Ginseng group ( P<0.01) .The tumor inhibitory rates in the group of Ginseng in SP cells and NSP cells were 43.04%,31.55%, respectively .The results of Western blot and immunohistochemical staining showed that Ginseng could inhibit the expression of Bcl-2 and induce the expression of Bax , the difference was statistically sig-nificant ( P<0.05 ,P<0.01 ) .Conclusion The tumorigenicity of SP cells is stronger than NSP cells .Ginseng can restrain the growth of the nude mouse transplanted tumor , and the mechanism may be associated with inducing cell apoptosis .关键词
侧群细胞/非侧群细胞/裸鼠移植瘤/人参Key words
side population cells/non-side population cells/nude mice transplantated tumor/Ginseng分类
医药卫生引用本文复制引用
钱军,刘元,郭成旭,朱超,承泽农..人参对胃癌 SGC-7901侧群细胞裸鼠移植瘤生长及 Bcl-2、Bax表达的影响[J].安徽医科大学学报,2016,51(6):773-777,5.基金项目
国家自然科学基金资助项目 （）
