安徽医科大学学报2016，Vol.51Issue(6)：809-812,813,5.
碳青霉烯耐药肺炎克雷伯菌耐药机制及分子流行病学研究
Analysis of Carbapenem-resistant Klebsiella pneumoniae molecular epidemiology and antibiotic resistance gene
摘要
Abstract
Objective To investigate the molecular epidemiology characteristics and drug resistance genes of imi -penem-resistant Klebsiella pneumoniae isolated .Methods A total of 44 strains of Carbapenem -resistant Klebsiella pneumoniae were collected and identified .Conventional drug susceptibility test was made by Vitek 2 compact auto-matic microbe susceptibility analyzer appraisal .Susceptibility testing for antibiotics was performed by the disc diffu-sion method carbapenemase production was confirmed by modified Hodge test and MBL production by IPM /IPM-EDTA combined disc test .The resistant genes were detected by PCR .Molecular epidemiology characteristics were analyzed by enterobacterial repetitive intergenic consensus sequence ( ERIC)-polymerase chain reaction ( PCR) .Re-sults A total of 44 strains of bacteria showed a high level resistance to routine antibiotics including carbapenems , penicillins , cephalosporin and aztreonam .A total of 41 strains were positive in modified Hodge test and all the 44 strains were negative to enzyme detection test .PCR result showed that 42 strains were of KPC-2 type, none of other gene types.ERIC-PCR result showed that 44 strains of Klebsiella pneumoniae were divided into 14 types, and the main type was type I .18 strains belonged to type I from ICU and neurosurgery .Conclusion Klebsiella pneumoniae that resists to carbapenem shows high levels of resistance to routine antibiotic in our hospital .Strains of carbapenem resistant Klebsiella pneumoniae appear , KPC-2 is the main reason to cause the bacterial resistance to carbapenems . Frequent transfer treatment of the patients among ICU and other clinical departments is the primary factor of carbap -enem-resistant Klebsiella pneumonia epidemic in the entire hospital .关键词
肺炎克雷伯菌/碳青霉烯酶/ERIC-PCR/KPC-2Key words
Klebsiella pneumoniae/carbapenemases/ERIC-PCR/KPC-2分类
医药卫生引用本文复制引用
储雯雯,刘周,杨凯,管世鹤..碳青霉烯耐药肺炎克雷伯菌耐药机制及分子流行病学研究[J].安徽医科大学学报,2016,51(6):809-812,813,5.基金项目
国家自然科学基金 （）
