安徽医科大学学报2016，Vol.51Issue(7)：978-983,984,7.
长链非编码 RNA SPRY4－IT1对膀胱癌生长的影响
Long non-coding RNA SPRY4-IT1 inhibited the progression of bladder cancer
摘要
Abstract
Objective To investigate the effect of long non -coding RNA (LncRNA) SPRY4 intronic transcript 1 (SPRY4-IT1) on the cell progression of bladder cancer .Methods To detect the SPRY4-IT1 expression by qRT-PCR and to analyze the correlation between its expression and clinical pathological features in the specimens and cells of bladder cancer .The specific small interfere RNA (si-SPRY4-IT1) was transfected into two bladder cancer cell lines respectively,and the transfection efficiency was evaluated by qRT -PCR.Then the CCK-8 assay, MTT as-say,migration assay, ELISA and flow cytometry assay were used to detect cell proliferation ,migration and apoptosis. Results The SPRY4-IT1 expression was obviously up-regulated in 68.33% of cancer samples (P =0.026 3) and related to bladder cancer histological grade (P <0.05) and TNM stage (P <0.001).The SPRY4-IT1 expression was obviously increased in 5637 (P =0.002 29) and T24 (P =0.000 12) .The expression of SPRY4-IT1 could be down-regulated by si-SPRY4-IT1 (P <0.001).Si-SPRY4-IT1 could remarkably inhibit the proliferation ( P <0.001),suppress imigration(P <0.01) and increase apoptosis(P <0.01).Conclusion SPRY4-IT1 is up-regu-lated in bladder cancer tissues and cells .SPRY4-IT1 plays an oncogene role in promoting cell growth of bladder cancer.关键词
膀胱癌/长链非编码 RNA/SPRY4-IT1/细胞增殖/细胞迁移/细胞凋亡Key words
bladder cancer/long non-coding RNA/SPRY4-IT1/cell proliferation/cell imigration/cell apoptosis分类
医药卫生引用本文复制引用
陈明伟,黎健发,庄成乐,刘宇辰,陈志聪,何安邦,张巧霞,黄卫人,蔡志明..长链非编码 RNA SPRY4－IT1对膀胱癌生长的影响[J].安徽医科大学学报,2016,51(7):978-983,984,7.基金项目
国家重点基础研究发展计划(973计划)(2014CB745200) ,国家自然科学青年基金(81402103) ,国家高技术研究发展计划(863计划)(2014AA020607) ,广东省自然科学基金(2014A030313717) ,深圳市科技计划项目(JCYJ20150330102720130、GJHZ20150316154912494) （973计划）
