安徽医科大学学报2016，Vol.51Issue(7)：1066-1070,5.
血清 AFP、AFP－L3联合检测在原发性肝癌中的诊断价值
Diagnosis value of AFP and AFP-L3 combined detection in hepatocellular carcinoma
摘要
Abstract
Objective To explore the diagnostic value of alpha fetoprotein (AFP), alpha fetoprotein L3(AFP-L3) and ratio of AFP-L3 to AFP in hepatocellular carcinoma(HCC).Methods In 354 patients, 110 patients with hep-atocellular carcinoma(HCC) and 244 patients with benign liver disease (BLD)(including 94 patients with hepati-tis, 126 patients with liver cirrhosis and 24 patients with benign liver tumors).AFP and AFP-L3 which was separa-ted by affinity adsorption centrifugal tube were measured by electrochemiluminescence immunoassays , following cal-culation ratio of AFP-L3 to AFP.Results In 110 patients with HCC, the patients of AFP <20 ng/ml, AFP 20 ~200 ng/ml and AFP >200 ng /ml were 32 cases, 30 cases and 48 cases respectively.In 244 patients with BLD, the patients of AFP <20 ng/ml were 196 cases.Levels of AFP, AFP-L3 and ratio of AFP-L3 to AFP in patients with HCC were significantly higher than that in patients with BLD (P <0.01).According to ROC curve, areas under the curve(AUC) of AFP, AFP-L3 and ratio of AFP-L3 to AFP were 0.809, 0.819 and 0.800 respectively, and AUC of combination three markers was 0.829(P <0.01).When the cut-off values of ratio of AFP-L3 to AFP were 5%, 10% or 15%, the sensitivities were 59.1%, 50.9% or 40.9%, and the specificities were 86.1%, 94.7%or 98.4%.Conclusion Diagnostic value of combination AFP and AFP -L3 is higher than AFP in diagnosis of hepa-tocellular carcinoma.The best cut-off value of ratio of AFP-L3 to AFP is 5%.关键词
原发性肝癌/甲胎蛋白/甲胎蛋白异质体Key words
hepatocellular carcinoma/alpha fetoprotein/alpha fetoprotein L3分类
医药卫生引用本文复制引用
徐恩君,陈秋莉,李涛,郝丽,肖春红,徐元宏,周敏,徐胜前..血清 AFP、AFP－L3联合检测在原发性肝癌中的诊断价值[J].安徽医科大学学报,2016,51(7):1066-1070,5.基金项目
国家自然科学基金(30801088、81201488、81571572) ,卫生部应用研究项目,“高通量 ELISA 检测系统化、标准化系列研究”(28－1－50) （30801088、81201488、81571572）
