北华大学学报（自然科学版）2016，Vol.17Issue(4)：473-476,4.DOI:10.11713/j.issn.1009-4822.2016.04.010
人参皂苷CK转化复合菌群的筛选及条件优化
Selection of Composite Microbial System for the Transformation of Ginsenoside Compound K and Optimization of the Biotransformation Conditions
摘要
Abstract
Objective To select the composite microbial system R2A48, which could effectively transform ginsenoside compound K, from ginseng soil by the methods of TLC and HPLC based on synergic relationship. Method At present,the microbial transformation method is primarily used to produce ginsenoside compound K, and the selection of efficient strains is the first requirement. Results The optimal biotransformation conditions obtained by factor biotransformation experiment were as follows:initial pH value of 7. 0,substrate addition of 3%, revolution of 140 r/min, temperature of 35℃, and the duration of 14 days. Conclusion Compared with single strain,this study provides a new idea and method for the microbial transformation of ginsenoside compound K,thus has important significance in the production of ginsenoside.关键词
人参皂苷CK/微生物转化/复合菌群/条件优化Key words
ginsenoside compound K/microbial transformation/composite microbial system/optimization of conditions分类
生物科学引用本文复制引用
孙海明,倪萍,王宇星,石磊,邓光敏,朱梅..人参皂苷CK转化复合菌群的筛选及条件优化[J].北华大学学报（自然科学版）,2016,17(4):473-476,4.基金项目
吉林省人参转化关键技术研究与应用创新团队项目(20140519018JH) （20140519018JH）
