北华大学学报（自然科学版）2016，Vol.17Issue(4)：481-487,7.DOI:10.11713/j.issn.1009-4822.2016.04.012
电针对5月龄APP/PS1转基因小鼠海马组织及组织间液可溶性Aβ1-42水平短时作用的影响
Short-term Effects of Electroacupuncture Therapy on the Concentrations of Soluble Aβ1-42 in Hippocampus and Brain Interstitial Fluid in APP/PS1 Transgenic Mice with 5 Months Old
摘要
Abstract
Objective To investigate the effects of electroacupuncture ( EA ) therapy on the levels of soluble Aβ1-42 in hippocampus and brain interstitial fluid ( ISF ) in mice with early-stage alzheimer ’ s disease ( AD ) . Method APP/PS1 double transgenic male mice with 5 months old were randomly divided into the model group and the acupuncture group. Wild type mice were used as the normal control group. Acupuncture on “Baihui”,“Yongquan” or constraint of the same condition was the intervention method, and the hippocampus interstitial fluid was obtained by cerebral microdialysis. The concentrations of Aβ1-42 in the hippocampus and ISF of the mice in three groups were measured by Elisa at the 6th, 7th, 8th and 9th hour after the intervention. Results Compared with the control group, the concentrations of Aβ1-42 in hippocampus and ISF of the model group were significantly higher (P<0. 01). Compared with the model group,the concentration of Aβ1-42 in ISF of the acupuncture group decreased (P<0. 05),but the concentrations showed no statistical difference between the two groups (P>0. 05). At the 6~9th hour after therapy,the concentration of Aβ1-42 of ISF in the treatment group did not change with time. Conclusion EA therapy can decrease the concentration of soluble Aβ1-42 in hippocampus ISF in mice with AD.关键词
阿尔茨海默病/电针/早期干预/脑组织间液/微透析Key words
Alzheimer’ s disease/electroacupuncture/early intervention/ISF/microdialysis分类
医药卫生引用本文复制引用
步青云,高堂珂,高杨,薛卫国..电针对5月龄APP/PS1转基因小鼠海马组织及组织间液可溶性Aβ1-42水平短时作用的影响[J].北华大学学报（自然科学版）,2016,17(4):481-487,7.基金项目
国家自然科学基金项目(81273826) （81273826）
北京中医药大学自主选题项目(2015-JYB-XS120) （2015-JYB-XS120）
