北华大学学报(自然科学版)2016,Vol.17Issue(4):537-539,3.DOI:10.11713/j.issn.1009-4822.2016.04.026
黑果枸杞组培繁殖培养基选择
Selection on Medium of Tissue Culture and Propagation of Lycium ruthenicum Murr
摘要
Abstract
By the ratio of different concentrations of plant growth regulators method,the key technology of rapid propagation Lycium ruthenicum Murr was studied by tissue culture. The result showed:MS was suitable for germination and seedling growth of Lycium ruthenicum Murr. Good callus was formed from free-germ seedling in MS+1. 0 mg/L 6-BA+0. 1 mg/L IBA. There were more adventitious buds in MS+0. 4 mg/L 6-BA+0. 1 mg/L IBA. The culture medium for adventitious root induction was 1/2 MS+1. 5 mg/L IBA(98%). The survival rate amounted to 93% of tissue culture seedling transplant in mixed matrix of peat,vermiculite and perlite(2 ∶1 ∶1).关键词
黑果枸杞/组织培养/繁殖/培养基Key words
Lycium ruthenicum Murr/tissue culture/propagation/medium分类
农业科技引用本文复制引用
冀菲,唐晓杰,程广有..黑果枸杞组培繁殖培养基选择[J].北华大学学报(自然科学版),2016,17(4):537-539,3.基金项目
吉林省科技厅重点攻关项目(20140204005NY) (20140204005NY)