北京林业大学学报2016，Vol.38Issue(6)：94-100,7.DOI:10.13332/j.1000--1522.20150364
应用流式细胞术测定18个中国古老月季基因组大小
Estimation of genome size of eighteen Chinese old garden roses by flow cytometry
摘要
Abstract
Using Petroselinum crispum as the calibration standard, we used two different test processes to estimate the genome size of Chinese old garden rose. Using chromosome counting method, we also determined the ploidy level of three Chinese old garden rose cultivars, which are still in debate or have not been reported, to offer necessary support for estimating the genome size. Main results are as follows. 1 ) The Chinese old garden rose cultivar ‘Pingdong Yueji ’ is diploid ( 2 n=2 x=14 ) , while ‘Jünang ’ and ‘Mudan Yueji ’ are tetraploid ( 2 n =4 x =28 ) . 2 ) Significant differences were observed in six cultivars, but no significant differences in other twelve cultivars in both two test processes, in which the method II was more accurate and stable to estimate the genome size. Method I was easier in operating and more suitable for detecting ploidy level. 3 ) The genome size of 18 Chinese old garden rose cultivars (including diploid, triploid and tetraploid cultivars) ranged from 0. 62 pg to 0. 71 pg. The 2C DNA amounts of diploid cultivars ranged between 1. 34--1. 43 pg, and C-value varied from 0. 67 pg to 1. 43 pg. The 2C DNA amounts of triploid cultivars ranged between 1. 96--2. 05 pg, and C-value between 0. 65--0. 68 pg. The 2C DNA amounts of tetraploid varieties varied from 2. 49 to 2. 63 pg, and C-value from 0. 62 to 0. 66 pg. Diploid cultivars possess the largest genome, followed by the triploid ones, and <br> tetraploid ones the least. We selected the optimum pretreatment method for estimating the genome sizes of 18 Chinese old garden rose cultivars using flow cytometry. Our results offer theory for revealing the relationship between the origin and evolution of Chinese old garden rose, and lay a foundation for their genomic sequencing.关键词
中国古老月季/流式细胞术/基因组大小/倍性Key words
Chinese old garden rose/flow cytometry/genome size/ploidy分类
农业科技引用本文复制引用
武荣花,葛蓓蓓,王茂良,周燕,冯慧..应用流式细胞术测定18个中国古老月季基因组大小[J].北京林业大学学报,2016,38(6):94-100,7.基金项目
北京市公园管理中心科技项目(ZX2015019)。 （ZX2015019）
