北京林业大学学报2016，Vol.38Issue(6)：101-109,9.DOI:10.13332/j.1000--1522.20150349
利用荧光标记SSR绘制中国芍药品种分子身份证
Using the SSR with fluorescent labeling to establish SSR molecular ID code for cultivars of the Chinese herbaceous peony
摘要
Abstract
Herbaceous peony( Paeonia lactiflora Pall. ) is a perennial herbaceous plant which belongs to Sect. Paeonia in family Paeoniaceae. It is a traditional flower with 3000-year history and has hundreds of cultivars in China. However, it is difficult to distinguish cultivars accurately only by the traditional morphological identification method. Establishing the multi-level molecular identification techniques can improve the accuracy of cultivar identification. In order to develop a molecular identification method,we used the SSR technique to establish the molecular ID code of 66 cultivars of Chinese traditional herbaceous peony. In this research, 111 pairs of SSR primers with 84 from literatures and 27 self-designed, were labeled with 6% none-denaturing polyacrylamide gel electrophoresis and quadruple fluorescence capillary electrophoresis. Then 29 pairs of primers with high polymorphism and good repeatability were selected. The results showed that 223 polymorphic alleles were revealed by the 29 primer pairs,with an average of 7. 69 alleles per pair. The effective number of alleles ( Ne) was 3. 658 ± 0. 328 , Shannon diversity index ( I ) was 1. 371 ± 0. 104 , observed heterozygosity ( Ho ) was 0. 532 ± <br> 0. 045, expected heterozygosity (He) was 0. 645 ± 0. 036, and the range of PIC at 29 SSR loci of 66 cultivars was 0. 20-0. 84, 0. 60 on average. Based on the principle of distinguishing the most varieties using the minimum pairs of primers, 4 pairs of primers (Pmg153, Knu73, Pmg209, J38) were selected out. This study indicates that the 4 pairs of primers can identify the 66 varieties of Chinese traditional herbaceous peony and can successfully establish 66 molecular ID codes by different single digits and uppercase letters. It not only provides a rapid, accurate and efficient molecular identification method for herbaceous peony cultivars, but also contributes to genetic improvement of cultivars of Chinese traditional herbaceous peony and is significant for protection of the new varieties.关键词
芍药/SSR荧光标记/分子身份证/品种鉴定Key words
herbaceous peony/fluorescent labeled SSR marker/molecular identity/cultivar identification分类
园艺学与植物营养学引用本文复制引用
张嘉,刘爱青,张淑玲,解莹然,刘燕..利用荧光标记SSR绘制中国芍药品种分子身份证[J].北京林业大学学报,2016,38(6):101-109,9.基金项目
北京市科委计划项目(D161100001916004)。 （D161100001916004）
