北京林业大学学报2016，Vol.38Issue(6)：123-129,7.DOI:10.13332/j.1000--1522.20160105
HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法
A method of HPLC-MS/MS to determine chlorogenic acid and other three kinds of active components in Eucommia ulmoids
摘要
Abstract
Eucommia ulmoids is a kind of traditional Chinese herbal medicine. Aucubin (AU), chlorogenic acid (CA), geniposidic acid (GA) and geniposide (G) are important components in E. ulmoids. A HPLC-MS/MS method was established to determine these four target chemicals in E. ulmoids. Chromatographic conditions were: the chromatographic column XD-C18 was adopted, the mobile phase was 0. 1% formic acid aqueous solution and methanol, the flow rate was 0. 15 mL/min, and the column temperature was 30℃. Mass spectrometry conditions were: ESI ion as a pyrolysis source, the pyrolysis temperature was 280℃, the source voltage was 4 kV, sheath gas was nitrogen, flow rate was 30 psi, the atomization gas was helium, and the flow rate was 10 psi. The R2 of CA, AU, G and GA standard curve were 0. 993 5, 0. 994 2, 0. 996 9 and 0. 994 8, respectively. Lower limit of detection was from 7. 603 to 8. 853 pmol. Spiked recovery was from 91. 838% to 108. 326%. In E. ulmoids, the content of AU was 20. 552 ± 4. 032 ng/mg dry weight ( DW) , the content of GA was 6. 913 ± 0. 654 ng/mg DW, the content of CA was 25. 986 ± 3. 412 ng/mg DW, and the content of G was 0. 205 ± 0. 015 ng/mg DW. This method was also used to determine the contents of these four target chemicals in other seven plant species, and the results indicated that the method is effective and widely applicable.关键词
杜仲/绿原酸/桃叶珊瑚苷/京尼平苷酸/京尼平苷Key words
Eucommia ulmoids/chlorogenic acid/aucubin/geniposidic acid/geniposide分类
农业科技引用本文复制引用
李冉,齐芪,李赟,陈雪梅,盖颖..HPLC-MS/MS检测杜仲中绿原酸等4种活性成分的分析方法[J].北京林业大学学报,2016,38(6):123-129,7.基金项目
北京高等学校“青年英才计划”项目(YETP0755)、北京市自然科学基金项目(5141001)。 （YETP0755）
