动物医学进展2016，Vol.37Issue(6)：1-6,6.
高致病性猪繁殖与呼吸综合征病毒双重液相基因芯片检测方法的建立
Development of a Duplex xMAP Assay for Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
A duplex xMAP bead-based assay was developed for rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV)and differentiation of highly pathogenic strains (HP-PRRSV).The xMAP assay could simultaneously detect two target sequences,including the GP2 envelope protein gene of PRRS and the Nsp2 gene conserved region specific for HP-PRRSV strains.The assay detected 12 PRRSV strains used in this study and identified 7 strains as HP-PRRSV,without cross-reactions with 12 non-tar-get swine pathogens.The lower detection limit of the assay on PRRSV,HP-PRRSV strains was deter-mined as below 0.5 TCID50 per reaction,respectively.The inter-assay and intra-assay variances (CV%) were both lower than 10%.The assay was applied to test 55 suspected field samples and compared with a commercialized single-plex real-time RT-PCR method.The detection results of the two methods were 98.2% (54/55 )in consistency.The study provided a novel molecular method for rapid diagnosis of PRRSV infection.关键词
猪繁殖与呼吸综合征病毒/高致病性猪繁殖与呼吸综合征病毒/xMAP液相芯片Key words
Porcine reproductive and respiratory syndrome virus (PRRSV)/highly pathogenic PRRSV (HP-PRRSV)/xMAP LiquiChip分类
农业科技引用本文复制引用
陈茹,于晓璐,高小博,薛春宜,宋长绪,邱杨,刘志玲,王莹,段燕喻..高致病性猪繁殖与呼吸综合征病毒双重液相基因芯片检测方法的建立[J].动物医学进展,2016,37(6):1-6,6.基金项目
国家质量监督检验检疫总局科研项目（2013IK029）；广东省农业攻关项目 （）
