动物医学进展2016，Vol.37Issue(6)：13-17,5.
猪瘟病毒石门株在 ST 细胞连续传代后 E0基因变异分析
Variation Analysis of E0 Gene of Classical Swine Fever Virus Shimen Strain in Continuously Passed ST Cells
摘要
Abstract
In order to study genetic variation of classical swine fever virus Shimen strains,the primers of CS-FV E0 gene of Shimen strain were designed and synthesized according to the published sequence (AF092448.2).The total RNA of classical swine fever virus Shimen strain continuouly passed in ST cells used as a template,and E0 gene was amplified by RT-PCR every five passages of cultured swine fever vi-rus.E0 nucleotide and amino acid sequences were determined.Variabilities of primary,5,10,15,20 and 25 passage viruses were analyzed by using DNA Star software.The results showed that variation appeared in 630 nucleotide point of 10 passage virus,and 632 nucleotide point of 15 passage virus.By molecular biologi-cal methods,this research revealed that the E0 genes of CSFV Shimen strain were genetically stable after passages in the ST cells.关键词
猪瘟病毒/连续传代/ST细胞/E0基因/变异Key words
Classical swine fever virus/continuous passage/ST cell/E0 gene/variation分类
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孙永科,胡超英,林明星,陆欣然,杨玉艾..猪瘟病毒石门株在 ST 细胞连续传代后 E0基因变异分析[J].动物医学进展,2016,37(6):13-17,5.基金项目
国家自然科学基金项目 （）
