动物医学进展2016，Vol.37Issue(6)：22-25,4.
H3N2亚型猪流感病毒 HA 和 HA1蛋白的原核表达
Prokaryotic Expression of HA and HA1 Genes of H3N2 Swine Influenza Virus
摘要
Abstract
To express the HA and HA1 proteins of H3N2 swine influenza virus,the HA and HA1 genes were amplified by RT-PCR and cloned into prokaryotic expression vector pMAL-c5X.Recombinant plasmid pMAL-c5X-HA and pMAL-c5X-HA1 were transformed to E.coli Rosetta (DE3)and induced with IPTG. The HA and HA1 proteins were expressed successfully in the forms of soluble proteins and inclusion bodies.What′s more,both of the HA and HA1 proteins could be recognized by HA antibody of H3N2 sub-type,it revealed that the HA and HA1 proteins have good antigenicity.关键词
H3N2亚型猪流感病毒/HA蛋白/HA1蛋白/原核表达Key words
H3N2 swine influenza virus/HA protein/HA1 protein/prokaryotic expression分类
畜牧业引用本文复制引用
纪方晓,陈济铛,石庆伟,王衡,张桂红..H3N2亚型猪流感病毒 HA 和 HA1蛋白的原核表达[J].动物医学进展,2016,37(6):22-25,4.基金项目
现代农业产业技术体系建设专项资金项目 （）
