福建农业学报2016，Vol.31Issue(5)：449-454,6.
鳗鲡肠道产蛋白酶菌株的筛选、鉴定及酶学性质研究
Protease-producing Bacterium Isolated from Intestines of Anguilla anguilla
摘要
Abstract
A bacterial strain , MJ15 ,that secreted high amount of proteases was isolated on a casein plate culture from the intestines of A nguilla anguilla . Activity of the enzyme was determined by using a fishmeal as the substrate for the digestion .By means of morphological observations ,biolog identification ,and phylogenetic analysis on 16S rRNA sequences , MJ15 was identified as Lactococcus lactis subsp . The activity of the crude protease it secreted peaked at 40℃ and pH 5.0. The thermal and alikali stabilities of the protease were relatively poor .The metal ions , K+ , Na+ , Cu2+ , and Fe2+ , enhenced the enzymatic activity , while Zn2+ or pepstatin Aslightly inhibited it . The Km and Vmax of the enzyme were determined to be 8.77 mg・mL -1 and 53.48 μg・min-1 ・mL -1 , respectively .关键词
欧洲鳗鲡/肠道细菌/蛋白酶/降解鱼粉Key words
A nguilla anguilla/intestinal bacteria/protease/fishmeal degradation分类
生物科学引用本文复制引用
宋永康,黄薇,吕新,任丽花..鳗鲡肠道产蛋白酶菌株的筛选、鉴定及酶学性质研究[J].福建农业学报,2016,31(5):449-454,6.基金项目
福建省自然科学基金（2014J0101）；福建省科技计划项目---省属公益类科研院所基本科研专项 （）
