福建农业学报2016，Vol.31Issue(5)：475-479,5.
培养基渗透压对铁皮石斛原球茎生长和多糖含量的影响
Effect of Osmotic Pressureon Growth and Polysaccharide Accumulation of Dendrobium Candidum Protocorms on Culture Medium
摘要
Abstract
Effect of osmosis onthe growth of and polysaccharide accumulation in theprotocorms of Dendrobium candidum on a culture medium were studied . The liquid medium wasadded with mannitolat varied concentrations to alter theosmotic pressure for the experiment .The results showed that ,at low concentrations of mannitol ,the growth andpolysaccharide synthesis ofthe protocorms were enhanced , but significantly inhibited at high concentrations . During the cultivation , the vigor , growth , polysaccharidecontent , andyield of the protocormsincreased when the mannitol concentration in the medium was 0 -20 g ・ L -1 . After35d culturein the medium with 20 g・L -1 mannitol , the dry weight ,polysaccharide content ,andyield ofthe protocormspeaked with the maximized values of 17.30 g・L -1 ,361.52 mg・g -1 ,and 6 255.38 mg・L -1 ,respectively .However , when the mannitol concentration was raised to 30-40 g・L -1 , all of those indices hit a downward trend suggesting the high osmotic pressure could be detrimental to theprotocorm cultivation .关键词
铁皮石斛/原球茎/多糖/渗透压/甘露醇Key words
Dendrobiumcandidum/protocorm/polysaccharide/osmotic pressure/mannitol分类
农业科技引用本文复制引用
苏江..培养基渗透压对铁皮石斛原球茎生长和多糖含量的影响[J].福建农业学报,2016,31(5):475-479,5.基金项目
广西教育厅项目（2013YB207）；广西高校重点实验室桂西北特色资源研究与开发实验室资助项目（桂教科研［2006］6号）；国家级大学生创新创业训练计划项目 （）
