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三组耐低温厌氧菌群的种群结构分析

陈宏 聂毅磊 罗立津 贾纬 陈星伟

福建农业学报2016,Vol.31Issue(5):503-507,5.
福建农业学报2016,Vol.31Issue(5):503-507,5.

三组耐低温厌氧菌群的种群结构分析

Microbial Communities of Three Low-temperature Resistant Microflora from Animal Waste

陈宏 1聂毅磊 1罗立津 1贾纬 1陈星伟1

作者信息

  • 1. 福建省微生物研究所/福建省新药 微生物 筛选重点实验室,福建 福州 350007
  • 折叠

摘要

Abstract

To improve the efficiency of anaerobic , low‐temperature bioconversion of waste materials to methane ,3 groups of psychrophilic microflora were studied .Dried cow manure was collected from local cattle farms for an enrichment culture at 15℃ .From the culture ,Group #5 microflora was identified as a potential source for further investigation .The microbes of the group were inoculated in the waste water effluent from a pig farm to obtaine the low‐temperature resistant Group Y5S . The microbial communities of Y5S , # 5 , and YJ (a microflora group originated from pig farm sewer ) were compared .In all 3 groups ,microbes belong to the phylums of Firmicutes , Bacteroidetes ,Synergistetes ,Proteobacteria ,Spirochaetes ,and Verrucomicrobia were found . They shared 85 core microbiomes of Clostridia class in Firmicutes phylum , as indicated by the high‐throughput sequencing . At genus level ,these psychrophiles included Acetobacterium ,Citrobacter ,Clostridium ,Desulfobulbus ,Desulfomicrobium , Hydrogenophaga , Oscillospira , Parabacteroides , Ruminococcus , Sedimentibacter , Sphingomonas , Sulfurospirillum ,Syntrophomonas ,Trichococcus ,and Devosia .Synergistetes in the mocroflora of Y5S seemed to suggest that this kind of microorganism might play an important role in the biogas fermentation .

关键词

低温/厌氧发酵/沼气/细菌群落/高通量测序

Key words

low temperature/anaerobic fermentation/biogas/microbial community/high-throughput sequencing

分类

资源环境

引用本文复制引用

陈宏,聂毅磊,罗立津,贾纬,陈星伟..三组耐低温厌氧菌群的种群结构分析[J].福建农业学报,2016,31(5):503-507,5.

基金项目

福建省科技计划重大专项 ()

福建农业学报

OA北大核心CSTPCD

1008-0384

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