福建农业学报2016,Vol.31Issue(5):503-507,5.
三组耐低温厌氧菌群的种群结构分析
Microbial Communities of Three Low-temperature Resistant Microflora from Animal Waste
摘要
Abstract
To improve the efficiency of anaerobic , low‐temperature bioconversion of waste materials to methane ,3 groups of psychrophilic microflora were studied .Dried cow manure was collected from local cattle farms for an enrichment culture at 15℃ .From the culture ,Group #5 microflora was identified as a potential source for further investigation .The microbes of the group were inoculated in the waste water effluent from a pig farm to obtaine the low‐temperature resistant Group Y5S . The microbial communities of Y5S , # 5 , and YJ (a microflora group originated from pig farm sewer ) were compared .In all 3 groups ,microbes belong to the phylums of Firmicutes , Bacteroidetes ,Synergistetes ,Proteobacteria ,Spirochaetes ,and Verrucomicrobia were found . They shared 85 core microbiomes of Clostridia class in Firmicutes phylum , as indicated by the high‐throughput sequencing . At genus level ,these psychrophiles included Acetobacterium ,Citrobacter ,Clostridium ,Desulfobulbus ,Desulfomicrobium , Hydrogenophaga , Oscillospira , Parabacteroides , Ruminococcus , Sedimentibacter , Sphingomonas , Sulfurospirillum ,Syntrophomonas ,Trichococcus ,and Devosia .Synergistetes in the mocroflora of Y5S seemed to suggest that this kind of microorganism might play an important role in the biogas fermentation .关键词
低温/厌氧发酵/沼气/细菌群落/高通量测序Key words
low temperature/anaerobic fermentation/biogas/microbial community/high-throughput sequencing分类
资源环境引用本文复制引用
陈宏,聂毅磊,罗立津,贾纬,陈星伟..三组耐低温厌氧菌群的种群结构分析[J].福建农业学报,2016,31(5):503-507,5.基金项目
福建省科技计划重大专项 ()