法医学杂志2016，Vol.32Issue(3)：193-195,3.DOI:10.3969/j.issn.1004-5619.2016.03.008
PowerPlex®21试剂盒扩增后的分型图谱中Y片段丢失的识别
Recognition of Y Fragment Deletion by Genotyping Graphs after Amplified by PowerPlex?21 Detection Kit
汪三存 1丁美满 1魏晓林 1张涛 2姚斐1
作者信息
- 1. 嘉兴市公安局刑侦支队DNA实验室,浙江 嘉兴 314001
- 2. 嘉兴市公安局南湖区分局刑侦大队,浙江嘉兴 314000
- 折叠
摘要
Abstract
Objective To recognize the possibility of Y fragment deletion of Amelogenin gene intuitively and simply according to the genotyping graphs.Methods By calculating the ratio of total peak height of genotyping graphs, the statistics of equilibrium distribution between Amelogenin and D3S1358loci,Amelo-genin X-gene and Amelogenin Y-gene, and different alleles of D3S1358loci from 1968 individuals was analyzed after amplified by PowerPlex? 21 detection kit.Results Sum of peak height of Amelogenin X allele was not less than 60% that of D3S1358loci alleles in 90.8% female samples, and sum of peak height of Amelogenin X allele was not higher than 70% that of D3S1358loci alleles in 94.9% male samples.Conclusion The result of genotyping after amplified by PowerPlex? 21 detection kit shows that the possibility of Y fragment deletion should be considered when only Amelogenin X-gene of Amelo-geninis detected and the peak height of AmelogeninX-gene is not higher than 70% of the total peak height of D3S1358loci.关键词
法医遗传学/DNA指纹法/基因频率/Amelogenin基因Key words
forensic genetics/DNA fingerprinting/gene requency/Amelogenin gene分类
社会科学引用本文复制引用
汪三存,丁美满,魏晓林,张涛,姚斐..PowerPlex®21试剂盒扩增后的分型图谱中Y片段丢失的识别[J].法医学杂志,2016,32(3):193-195,3.
