广西科学2016，Vol.23Issue(2)：102-110,9.
溶菌酶触发的芽孢杆菌芽孢萌发及其异质性研究
Kinetics of Germination of Individual Bacillus Spores and its Heterogeneity Triggered by Lysozyme
摘要
Abstract
Objective]The heterogeneous germination and its mechanism of Baillus spores trig-gered by lysozyme were studied in order to look insight into the mechanism of spore germina-tion in general and provide new knowledge of inactivating bacterial spores.[Methods]The ki-netic of germinations of multiple individual decoated spores of Bacillus subtilis (Bs)and B. megaterium (Bm)triggered by lysozyme were followed by Raman spectroscopy and differentail interference contrast (DIC)microscopy.[Results]Higher concentrations of lysozyme and tem-peratures significantly speeded the germination of Bs decaoted spores and reduced the time Tlag , at which spores began release of the great major-ity of spores’1∶1 chelate of Ca2+ with dipico-linic acid (CaDPA),the period time of comple-ting the release of CaDPA (namedΔTrelease )and the time period of hydrolysis of the spore’s pep-tidoglycan cortex (named ΔTlys ).The ΔTrelease values of germination below 20℃ were 4 times more than that at 25℃.TheΔTrelease values of Bs spores with 4-fold-elevated level of SpoVA protein were basically similar to control strains, while spores lacking cortex-lytic enzymes exhibited notably higherΔTrelease values than wild-type spores.The values of Tlag ,ΔTrelease andΔTlys of spores treated by wet heat at 9 5℃ for 1 5 min were two times more than the untreated spores.Heterogeneous germinations were ob-served not only among individual decoated Bs spores,but also among Bs strains.The decoated Bm spores were more sensitive to lysozyme and its germinations of individual spores were less heterogeneous.[Conclusion]The germinations of decoated Bacillus spores triggered by lyso-zyme,on which the lysozyme concentration and temperature have a significant effect,are signif-icantly heterogeneous at the levels of single spores and strains,as well as of species.The cortex-lytic enzymes may be involved in the initiation of germination.关键词
拉曼光谱/微分干涉差成像/芽孢杆菌/芽孢萌发/溶菌酶/单细胞分析Key words
Raman spectroscopy/DIC imaging/Baillus/spore germination/lysozyme/single-cell analysis分类
生物科学引用本文复制引用
王桂文,张鹏飞,王晓春,陈欢君,Peter Setlow,Li Yongqing..溶菌酶触发的芽孢杆菌芽孢萌发及其异质性研究[J].广西科学,2016,23(2):102-110,9.基金项目
国家自然科学基金项目（11264004，31460035）资助。 （）
