广西科学2016，Vol.23Issue(2)：115-119,5.
不同营养方式下小球藻生长与光合作用的变化
Variation of Growth and Photosynthesis in Chlorella so-rokiniana Under Different Trophic Modes
摘要
Abstract
Objective]The variation of growth and photosynthesis of microalgae was studied and compared under autotrophic condition and hetero-and mixotrophic conditions with acetate as carbon source.[Methods]With Chlorella sorokiniana as material,the changes of growth and photosynthesis were reflected by determining the OD550 and the chlorophyll fluorescence from photosystem Ⅱ(PS Ⅱ).[Results]The growth rates of hetero-and mixotrophic conditions were significantly higher than that of autotrophic condition.It was only 1 .5 d for cells reaching sta-tionary phase under hetero- and mixotrophic conditions,but 9 d for cells under autotrophic condition.The parameters of chlorophyll fluo-rescence under autotrophic condition were signif-icantly higher than that under hetero-and mix-otrophic conditions,and that under mixotrophic condition were significantly higher than that un-der heterotrophic condition.The effective PS Ⅱquantum yield under mixo-and heterotrophic condition was reduced 21.5% and 98.1%,re-spectively,compared with that of autotrophic condition.The mRNA expression of Rubisco gene under mixotrophic condition were highest,and 3 .2-fold and 1 .8-fold of that under hetero-and autotrophic conditions,respectively.[Conclusion]The photosystems ofChlorella under mixotro-phic condition only slightly inhibited.In addition,the growth rate and maximal cell density un-der mixotrophic condition were higher than other conditions.Therefore,mixotrophic growth mode is suitable for large-scale cultivation.关键词
小球藻/自养/异养/混养/光合作用Key words
Chlorella sorokiniana/autotrophic/heterotrophic/mixotrophic/photosynthesis分类
农业科技引用本文复制引用
王超,孙春晓,乔洪金,丛超,王际英,张利民..不同营养方式下小球藻生长与光合作用的变化[J].广西科学,2016,23(2):115-119,5.基金项目
山东省优秀中青年科学家科研奖励基金项目（BS2013HZ018），国家自然科学基金青年基金项目（31201973），海洋生物产业---水生动物营养与饲料研发创新示范平台项目（201303003）和山东省科技发展计划项目（2014GHY115006）资助。 （）
