广西科学2016，Vol.23Issue(2)：120-124,5.
小球藻（Chlorellavulgaris）抗铵品系的紫外诱变选育
UV Mutagenesis and Screening for Ammonium Tolerant Strains of Chlorella vulgaris
摘要
Abstract
Objective]Breeding the new strains of Chlorella vulgaris that have a tolerance to high concentration of ammonium can efficiently avoid being eaten by the contamination animals such as rotifer,ciliate and so on during cultivation.[Methods]The original strain of Chlorella vulgaris was first mutated by exposing to UV light and then was screened by the high concen-tration of ammonium in media.[Results]Two mutants,B4 and C6,were acquired with better growth rate than the original one.The biomass of strains B4 and C6 after being cultivated for 12 d increased 41.18% and 9.54%,32.64% and 29.41%,37.74% and 22.85%,respectively, under different ammonium concentration,which was 800 mg/L,1 000 mg/L,and 1 200 mg/L. The Fv/Fm results also proved a significant improvement on their maximum light conversion efficiency.[Conclusion]The mutants B4 and C6 showed growing advantages under higher am-monium concentration,which may be gained from the enhancement of photosynthesis efficien-cy.关键词
小球藻/紫外诱变/抗高铵/光能转化效率Key words
Chlorella vulgaris/UV mutagenesis/high ammonium tolerant/Fv/Fm分类
农业科技引用本文复制引用
曹晓菲,丰圣伟,宋新伟,徐涤..小球藻（Chlorellavulgaris）抗铵品系的紫外诱变选育[J].广西科学,2016,23(2):120-124,5.基金项目
北海市科学研究与技术开发计划项目（201203024）资助。 （）
