广西植物2016，Vol.36Issue(6)：741-746,6.DOI:10.11931/guihaia.gxzw201306017
早熟禾细胞膜脂肪酸衍生化方法比较
Comparison of three derivatization methods for the determination of fatty acid profiles in cell membranes for bluegrass (Poa pratensis)
摘要
Abstract
The characteristic difference in bio-membrane in plant cells makes it difficult to use the same esterification method to determine the fatty acids contents,which are the important component of cell biological membrane. In order to establish a simple and reliable esterification method,in the present study,we isolated four kinds of cell membranes,chlo-roplasts,mitochondria,vacuoles and cytoplasm membrane respectively. Their fatty acids were extracted,esterified and de-termined by gas chromatography (GC) following three different methods. The results showed that both BF3-methanol and HCl-methanol esterification methods could not effectively esterify some of the fatty acids in mitochondria. Compared with the two approaches,acetyl chloride method could trans-esterify fatty acids completely and efficiently. Our data on fatty acids analysis with GC determination indicated that the third method had a high repeatability and accuracy. The current study provides valuable information for fatty acid determination on the plant cell membranes from different species.关键词
细胞膜/脂肪酸/气相色谱法/氯乙酰Key words
cell membrane/fatty acid/gas chromatography/acetyl chloride分类
农业科技引用本文复制引用
王维,黄亚亚,王娜,崔浪军..早熟禾细胞膜脂肪酸衍生化方法比较[J].广西植物,2016,36(6):741-746,6.基金项目
国家科技部科技惠民计划项目(2012GS610102)[Supported by the National Planning Program Beneficial to People of Ministral of Science and Technology(2012GS610102)]。 （2012GS610102）
