广西植物2016，Vol.36Issue(6)：752-757,6.DOI:10.11931/guihaia.gxzw201405050
拳参中没食子酸和总鞣质的含量测定
Determination of gallic acid and total tannins in Polygonum bistorta
摘要
Abstract
To further complement and perfect about content of Chinese Pharmacopoeia, the major component-gallic acid and total tannins in Polygonum bistorta were determined by high performance liquid chromatography (HPLC) and ultravio-let (UV) spectrophotometric methods for the essay, respectively. The HPLC condition for determination of gallic acid with Hypersil GOLD phenyl column (250 mm × 4.6 mm, 5 μm) and a mobile phase consisted of methanol and 0.1% phosphoric acid by gradient elution at a flow rate of 1.0 mL•min-1. The detection wavelength was 276 nm by wavelength scanning from 190-400 nm. On the other side, the total tannins were determined by Casein Method according to appendices XB in Phar-macopoeia of the People’s Republec of China (2010 ed), and the results were obtained by calculating the contend chang of total tannins before and after combination with the casein. The results showed that the linear range was 0.051-1.02 μg for gallic acid, the average recovery rates were 99.1%, 100.3%, 101.9%, and RSDs were 2.1%, 0.8%, 2.0%, respectively. The linear range was 2.09-10.48 μg for the total tannins, the average recovery rates were 102.2%, 100.2%, 102.1%, and RSDs were 1.3%, 1.4%, 1.0%, respectively. The investigation indicated that both methods had a preferable result for meth-odology validation. On the other hand, the essay also researched the P. bistorta from Guizhou and Sichuan, respectively, and the results showed that the conten of gallic acid and total tannins in P. bistorta had a great difference both Guizhou and Sichuan, the gallic acid and total tannins in P. bistorta from Guiyang were 1.53, 1.37, 1.12 mg•g-1 and 5.01, 4.87, 5.23 mg•g-1, respectively. And gallic acid and total tannins in P. bistorta were 2.72, 3.35, 2.46 mg•g-1 and 5.01, 4.87, 5.23 mg•g-1 from Chengdu, respectively. A preliminary analysis on this issue was made with many factors, as far as we know, the deciding factor should be the altitude. The references showed that the best condition for P. bistorta was an elevation from 800 to 3 000 m, while, the altitude of Guiyang at range of 800 to 1 600 m and altitude at range of 1 000 to 3 000 m for Chengdu. So, we belived that altitude of Chengdu was more amenable to the efficient growth of the P. bistorta, which futher caused the contents of gallic acid and total tannins relatively higher than Guiyang. The results provide a theory basis for pharmaceu-tical company to select P. bistorta for producing drugs. The research revealed that those methods were rapid, simple and re-producible and could be used to determine gallic acid and total tannins in P. bistorta. However, methods were the first to be used for determine gallic acid and total tannins in P. bistorta simultaneously, these data provide a basis for the further com-plement and perfect about content of Chinese Pharmacopoeia.关键词
拳参/没食子酸/鞣质/高效液相色谱Key words
Polygonum bistorta/gallic acid/tannin/HPLC分类
农业科技引用本文复制引用
娄华勇,刘亚洲,张文芳,潘卫东..拳参中没食子酸和总鞣质的含量测定[J].广西植物,2016,36(6):752-757,6.基金项目
中国科学院“西部之光”人才培养计划项目[Supported by the West Light Foundation of Chinese Academy of Sciences]。 （）
