河南中医2016，Vol.36Issue(6)：991-993,3.DOI:10.16367/j.issn.1003-5028.2016.06.0414
健肾助孕方对着床障碍小鼠雌孕激素及子宫HOXA10表达的影响
The Influence of Cai's Jianshen Zhuyun Decoction on Estrogen and Progesterone and Womb HOXA10 Expression of Mice with Imbed Barrier
庄梦斐 1袁雪菲 1张婷婷 1曹阳 1谭丽 1金毓莉 2崔金刚1
作者信息
- 1. 上海中医药大学附属岳阳中西医结合医院,上海200437
- 2. 上海市第一人民医院,上海200080
- 折叠
摘要
Abstract
Objective:To observe the influence of Cai's Jianshen Zhuyun Decoction (CJSZYD)on estrogen and progesterone and womb HOXA10 expression of mice with imbed barrier.Methods:Thirty pregnant mice were randomly divided into the model group with 3 cases,the group of low-dosage CJSZYD with 6 cases,the group of high-dosage CJSZYD with 6 cases,the progesterone group with 7 cases and the normal control group with 8 cases.From the 1 st day of pregnancy,any one in the model group and the normal control group were given 0.2 mL of normal saline for gavage each day;Any one in the progesterone group were given 0.1 mL of progesterone fluid for subcutaneous injection each day;Any one the group of low-dosage CJSZYD and the group of high-dosage CJSZYD were given 0.2 mL of decoction for gavage.From the 4th day of pregnancy,mifepristone was used for subcutaneous injection in order to induce the mouse's model with imbed barrier.On the 8th day of pregnancy,the mice were killed.Then serum estrogen and progesterone concentration was detected by using ELISA method;Womb HOXA10 expression and protein content were measured by using immunohistochemical method and Western blot method.Results:Compared with the model group,serum estrogen and progesterone concentration of the group of low-dosage CJSZYD and the group of high-dosage CJSZYD were obviously increased,and the differences between them had statistical significance (P＜O.05).Compared with the model group,Womb HOXA10 expression the group of high-dosage CJSZYD and the progesterone group obviously increased,and the differences between them had statistical significance (P＜0.05).Womb HOXA10 protein contents of the group of low-dosage CJSZYD and the group of high-dosage CJSZYD and the progesterone group were obviously higher than those of the model group,and the differences between them had statistical significance (P＜0.05).Conclusion:CJSZYD can increase and regulate estrogen and progesterone levels and womb HOXA10 expression of mice with imbed barrier,thus improving endometrial receptivity and embryo implantation.关键词
健肾助孕方/着床障碍/雌激素/孕激素/HOXA10/小鼠Key words
Cai's Jianshen Zhuyun Decoction (CJSZYD)/imbed barrier/estrogen/progesterone/HOXA10/mice分类
医药卫生引用本文复制引用
庄梦斐,袁雪菲,张婷婷,曹阳,谭丽,金毓莉,崔金刚..健肾助孕方对着床障碍小鼠雌孕激素及子宫HOXA10表达的影响[J].河南中医,2016,36(6):991-993,3.
