激光生物学报2016，Vol.25Issue(3)：214-222,9.DOI:10.3969/j.issn.1007-7146.2016.03.005
核壳结构 CdS-TiO2纳米颗粒体外光催化灭活白血病 HL60细胞实验研究
Photocatalytic Inactivation of Core-Shell CdS-TiO2 Nanoparticles on Leukemia HL60 Cancer Cells
摘要
Abstract
The photocatalytic inactivation of core-shell cadmium sulfide-titanium dioxide nanoparticles (CdS-TiO2 nano-particles)on HL60 cells and its mechanism were researched in this paper.Core-shell cadmium sulfide-titanium dioxide (CdS-TiO2 )nanoparticles were synthesized by ultrasonic method.The photocatalytic inactivation of these core-shell nan-oparticles on HL60 cells were analyzed by CCK-8 assay.Its mechanism were investigated using reactive oxygen species (ROS)generation and fluorescence emission spectrum analysis.Results of cell experiment showed that,in comparison with the cell group of CdS,viability of cells treated with CdS-TiO2 increased from 28.5% to 80%,with the increment of TiO2 shell thickness in the darkroom conditions.However,under light exposure,their viability decreased sharply.More than 60% cells were killed in each group treated by CdS-TiO2 .Among these samples,cell groups treated by CdS-TiO2 sample 0.6 showed the highest cell inactivated efficiency of 95%.According to the results of ROS generation and FS a-nalysis,it could be concluded that the existence of CdS resisted the recombination of photo-induce holes and electrons, enhancing the photocatalytic activity of TiO2 shell and eventually resulting in superior cell killing effect.关键词
CdS-TiO2 纳米颗粒/光动力疗法(PDT)/白血病 HL60 细胞Key words
TiO2 nanoparticles/photodynamic therapy/leukemia HL60 cells分类
临床医学引用本文复制引用
张卿,罗有焕,鲁丽,陈丽,艾保全,熊建文..核壳结构 CdS-TiO2纳米颗粒体外光催化灭活白血病 HL60细胞实验研究[J].激光生物学报,2016,25(3):214-222,9.基金项目
国家自然科学基金（61072029）；广州市科技计划资助项目 （）
