昆虫学报2016，Vol.59Issue(5)：568-572,5.DOI:10.16380/j.kcxb.2016.05.012
绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力
Chlorogenic acid enhances the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV)
摘要
Abstract
[Aim] To identify the effects of chlorogenic acid (CA) on the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV) so as to provide information for the prevention and control of the gypsy moth,L.dispar.[Methods] CA + LdNPV and LdNPV were bioassayed using diet plus method,to determine the dose and time response of the 2nd instar larvae of L.dispar.[Results] CA + LdNPV and LdNPV differed significantly in dose and time response when they were tested against the L.dispar larvae.The median lethal concentration (LC50) value of CA + LdNPV to the 2nd instar larvae was 161.8 OBs/μL (the 95％ confidence limit was 105.6-235.3OBs/μL),and that of LdNPV was 264.4OBs/ μL (the 95％ confidence limit was 178.6-384.0OBs/μL).CA + LdNPV was more virulent than LdNPV against L.dispar larvae.The median lethal time (LT50) value of CA + LdNPV was shorter than that of LdNPV.The LT50 values of CA + LdNPV and LdNPV were 9.9 and 12.3 days,respectively,at the concentration of 590 OBs/μL of LdNPV,and 6.9 and 8.0 days,respectively,at the concentration of 5 900 OBs/μL.The CA lowered the LC50 value and shortened the LT50value of LdNPV against the 2nd instar larvae of L.dispar.[Conclusion] Chlorogenic acid enhances the virulence of LdNPV against the 2nd instar larvae of L.dispar,and its action mechanism needs further study.关键词
舞毒蛾/核型多角体病毒/绿原酸/致病力/致死中浓度/致死中时间Key words
Lymantria dispar/LdNPV/chlorogenic acid/virulence/median lethal concentration/median lethal time分类
生物科学引用本文复制引用
刘海晶,段立清,李海平,冯淑军,张波波..绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力[J].昆虫学报,2016,59(5):568-572,5.基金项目
国家自然科学基金项目(31160161) （31160161）
林业公益性行业科研专项项目(201504302) （201504302）
