临床眼科杂志2016，Vol.24Issue(3)：260-263,4.DOI:10.3969/j.issn.1006-8422.2016.03.023
氧化低密度脂蛋白通过 TLR4介导小鼠RPE 细胞炎症反应
Oxidized low-density lipoprotein induces mouse retinal pigment epithelium cell inflammation via toll-like re-ceptor 4 Activation
摘要
Abstract
Objective To study the inflammatory secretion of mouse retinal pigment epithelium ( RPE) induced by oxidized low-density lipoprotein (OX-LDL) and the potential role of Toll-like receptor 4 (TLR4) in this process.Methods RPE cells of wild and gene knock-out ( TLR4-/-) C57 mice were primary cultured in vitro with enzyme digestion .The cultured RPE cells were identified with immunocytochemical staining .RPE cells were incubated with 10-100 mg/mL n-ative-LDL (n-LDL) or OX-LDL for 24h.IL-6 and IL-1βprotein in the supernatant was measured by enzyme-linked im-mune sorbent assay (ELISA).Western blot analysis and reverse transcription polymerase chain reaction (RT-PCR) were used to detect TLR4 protein and mRNA levels in RPE cells .Results 50 and 100 mg/mL OX-LDL treatment increased IL-6 and IL-1βsecretion in wild mouse RPE cells , with TLR4 mRNA and protein expression activated .There was no signifi-cant IL-6 and IL-1βsecretion increase in TLR4-/-mouse RPE cells.Conclusion Through TLR4 upregulation, OX-LDL induces inflammation promotion in cultured mouse RPE cells .关键词
视网膜色素上皮细胞/炎症/Toll样受体4/低密度脂蛋白Key words
Retinal pigment epithelium/InflammationLToll-like receptor 4/Oxidized low-density lipoprotein引用本文复制引用
舒婉婷,吴星伟,尹莉莉..氧化低密度脂蛋白通过 TLR4介导小鼠RPE 细胞炎症反应[J].临床眼科杂志,2016,24(3):260-263,4.基金项目
国家自然科学基金 （）
