临床眼科杂志2016，Vol.24Issue(3)：264-267,4.DOI:10.3969/j.issn.1006-8422.2016.03.024
大鼠的两种干细胞向光感受器细胞和视网膜色素上皮细胞分化的实验研究
Differentiation of rat two-derived stem cells into photoceptors and RPE cells
许卓再 1王婧 1毛毳 1余烁 1卢弘 1董方田2
作者信息
- 1. 100020 北京，首都医科大学附属北京朝阳医院
- 2. 100730 北京，中国医学科学院 中国协和医科大学附属北京协和医院眼科
- 折叠
摘要
Abstract
Objective To explore method to isolate and culture bone marrow mesenchymal stem cells (MSCs) and adipose-derived stem cells(ADSCs), and in-vitro to induce them into photoceptors and RPE cells .To provide material ba-sis for the clinical treatment of retinal diseases .Methods The MSCs and ADSCs were cultured by adhering to the flask surface and purified by continual passage .Surface antigens including CD45、CD90、CD49d、CD106 were indentified by flow cytometry.MSCs and ADSCs were induced to differentiate by extracted liquid of retina ,EGF, taurine and retinoic acid (RA)respectively.Flow cytometry was used for quantifying the expression of rhodopsin , CK and S-100 and the induction efficacy.Results Purified from passage 1 to 5, MSCs phenotypes were: CD45(1.8%~7.5%), CD90(83.8%~95. 7%), CD49d(6.6%~24.8%)and CD106(15.7%~32.2%); ADSCs phenotypes were: CD45(0.8%~9.3%), CD90(84.7%~94.8%), CD49d(16.8%~31.0%)and CD106(8.3%~22.2%).In all the passages, there was a lower CD49d percentage than CD106 in MSCs and a higher CD49d percentage than CD106 in ADSCs contrarily.The induc-tion efficacy of MSCs was 55.9%~70.0%for rhodopsin, 49.5%~72.0%for CK and 25.4%~34.0%for S-100 and of ADSCs was 19.6%~31.1%for rhodopsin, 31.0%~79.3%for CK and 27.3%~50.7%for S-100.Conclusion It is suggested that MSCs and ADSC have potential to differentiate into photoceptors and RPE cells .Both of them may represent sources of stem cells transplantation for retinopathy.关键词
骨髓间充质干细胞/脂肪干细胞/分化/光感受器细胞/视网膜色素上皮细胞Key words
Bone marrow mesenchymal stem cells/Adipose-derived stem cells/Differentiation/Photoceptor/Retinal pigment epithelium引用本文复制引用
许卓再,王婧,毛毳,余烁,卢弘,董方田..大鼠的两种干细胞向光感受器细胞和视网膜色素上皮细胞分化的实验研究[J].临床眼科杂志,2016,24(3):264-267,4.
