中国临床保健杂志2016，Vol.19Issue(4)：400-403,4.DOI:10.3969/J.issn.1672-6790.2016.04.022
血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子－1表达的影响
Effect of Xuebijing on expression of ICAM-1 in the lung tissue of rats with acute lung injury induced by lipopo-lysaccharide
张之龄 1沈琪琦 1朱健 1童小文 1徐兵 1周吴刚1
作者信息
- 1. 上海交通大学医学院附属第九人民医院急诊科，上海 200011
- 折叠
摘要
Abstract
Objective To observe the effect of Xuebijing on expression of intercellular adhesion molecule-1 (ICAM-1)in the lung tissue of rats with acute lung injury induced by lipopolysaccharide.Methods Forty-five Wistar rats were randomly divided into 3 groups by random number table method:(1)control group(n =15),(2)LPS group (n =15),(3)Xuebijing group(XBJ group)(n =15).Injection of LPS(5 mg/kg)into femoral vein to reproduce ALI model in LPS group and XBJ group.Male Wistar rats of XBJ group were given XBJ 4 mL/kg 30 min before the intrave-nous injection of LPS,whereas control group and LPS group were given equivalent volume normal saline.Five rats in each group were killed at 6 h,12 h and 24 h,which were divided into subgroup I,Ⅱ,Ⅲ.The pathomorphism changes of lung tissue were observed by light microscope,and expression of ICAM-1 in pulmonary was examined by immunohisto-chemistry.Results Expression of ICAM-1 were gradually increased with experimental time prolonged and reached the peak at 24h in LPS group after establishing model.ICAM-1 were significantly lower in XBJ group than LPS group at each time points (P <0.05).Compared to LPS group,the pathological change was significantly reduce in XBJ group under microscope.Conclusion Xuebijing can inhibit the expressions of ICAM-1 and reduce acute lung injury induced by li-popolysaccharide in the lung tissue of rats.关键词
急性肺损伤/脓毒症/胞间黏附分子 1/大鼠,WistarKey words
Acute Lung injury/Sepsis/Intercellular adhesion molecule-1/Rats,Wistar分类
临床医学引用本文复制引用
张之龄,沈琪琦,朱健,童小文,徐兵,周吴刚..血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子－1表达的影响[J].中国临床保健杂志,2016,19(4):400-403,4.
