环境与职业医学2016，Vol.33Issue(6)：536-541,6.DOI:10.13213/j.cnki.jeom.2016.16210
氟化钠对人原代成骨细胞p21基因组蛋白乙酰化及表达的影响
Effects of Sodium Fluoride on Histone Acetylation and Expression of p21 Gene in Human Primary Osteoblasts
摘要
Abstract
[Objective] To observe the effects of sodium fluoride on the levels of histone acetylation,mRNA transcription,and protein expression of p21 gene in human primary osteoblasts and provide evidence for a potential molecular mechanism of skeletal fluorosis.[Methods] Primary osteoblasts were treated with 0,125,250,500,and 1000 μmol/L sodium fluoride for 72 h.The levels of histone acetylation in transcription regulatory region (ChIP1 region) and coding region (ChIP2 region) of p21 gene were detected by quantitative chromatin immuno precipitation.The levels of p21 mRNA transcription were detected by real-time quantitative PCR.The expression levels of P21 protein were detected by Western blot.[Results] After human osteoblasts were treated with 0,125,250,500,and 1000 μmol/L sodium fluoride,in ChIP1 transcription regulatory region of p21 gene,the levels of histone acetylation of H3K9 were 1.998 ± 0.085,1.644 ± 0.162,1.381 ± 0.069,1.280 ± 0.129,and 1.040 ± 0.270,respectively(F=15.757,P＜ 0.05);the levels of histone acetylation of H3K14 were 1.344 ± 0.068,1.248 ± 0.070,1.140 ± 0.090,1.040 ± 0.116,and 0.770 ± 0.059,respectively (F=21.284,P＜0.05);the levels of histone acetylation of H4K 16 were 1.330 ± 0.084,1.251 ± 0.085,1.087 ±0.044,0.854 ± 0.070,and 0.788 ± 0.051,respectively (F=36.429,P＜0.05).Among the groups treated with different concentrations of fluoride,the levels of histone acetylation of H3K9,H3K14,and H4K16 in ChIP2 coding region were not statistically different(F=0.084,0.206,and 0.500,all Ps ＞0.05).The levels of p21 mRNA transcription were 2.65 ± 0.35,1.93 ± 0.48,0.88 ± 0.17,0.82 ±0.09,and 0.67 ± 0.16,respectively (F=3.90,P＜ 0.05).The levels of P21 protein expression were 1.62 ± 0.06,1.02 ± 0.08,0.84 ±0.07,0.26 ± 0.05,and 0.07 ± 0.03,respectively (F=281.58,P＜0.05).[Conclusion] Fluoride could reduce the levels of histone acetylation of p21 gene transcription regulatory region in human primary osteoblasts,then suppress the expression of p21 mRNA transcription and the protein,which might be one of the important molecular mechanisms of active cell proliferation in skeletal fluorosis.关键词
氟/人成骨细胞/21基因/组蛋白乙酰化Key words
fluoride/human osteoblast/p21 gene/histone acetylation分类
医药卫生引用本文复制引用
游同钊,廖玉丹,严威敏,于春,罗鹏,潘雪莉..氟化钠对人原代成骨细胞p21基因组蛋白乙酰化及表达的影响[J].环境与职业医学,2016,33(6):536-541,6.基金项目
国家自然科学基金(编号:81260418) （编号:81260418）
贵州省优秀科技教育人才省长基金(编号:黔省专合字[2011]54号) （编号:黔省专合字[2011]54号）
