环境与职业医学2016，Vol.33Issue(6)：562-566,5.DOI:10.13213/j.cnki.jeom.2016.16209
镉诱导HK-2细胞信号通路的蛋白磷酸化变化及缝隙连接蛋白43的作用
Alteration of Protein Phosphorylation and Role of Connexin 43 in HK-2 Cells Following Cadmium Exposure
摘要
Abstract
[Objective] To investigate the alteration of protein phosphorylation of signaling pathways and the role of connexin 43 (Cx43) in human renal proximal tubular cells following cadmium (CdCl2) treatment.[Methods] Human renal proximal tubular cell lines (HK-2) were exposed to 1 tmol/L CdCl2 for 10 days.Cx43 was silenced by RNA interference technique(siRNA).Western blot was used to detect protein expression of Cx43.The protein phosphorylation of signaling pathways was determined by phosphor explorer antibody array.[Results] After HK-2 cells exposed to CdCl2 for 10 days,61 phosphorylation proteins were altered,belonging to ErbB,mitogen-activated protein kinase (MAPK),T cells receptor,and B cells receptor signaling pathways.Decreased proliferation (P＜ 0.05) and elevated apoptosis (P ＜ 0.05) in HK-2 cells were observed.After Cx43 was silenced prior to the exposure,56 phosphorylation proteins were altered when compared with the control group,which belonged to Focal adhesion,ErbB,and MAPK signaling pathways.However,apoptosis did not change (P ＞ 0.05) with decreased cell proliferation (P ＜ 0.05).Compared with the CdCl2 exposure group,102 altered phosphorylation proteins involved in Focal adhesion,ErbB,MAPK,Apoptosis,and Chemokine signaling pathways were identified with decreased apoptosis (P ＜ 0.05) and no change in cell proliferation (P＞ 0.05) in the Cx43 silencing posterior to CdCl2 exposure group.[Conclusion] An extended cadmium exposure of HK-2 cells could decrease cell proliferation,increase apoptosis,and affect ErbB,MAPK,T cells receptor,and B cells receptor signaling pathways.Down-regulated expression of Cx43 may regulate the proliferation and apoptosis of cells by changing the protein phosphorylation of signaling pathways such as Focal adhesion,ErbB,MAPK,Apoptosis,and Chemokine.关键词
氯化镉/人肾近曲小管上皮细胞/蛋白磷酸化/缝隙连接蛋白43/siRNAKey words
cadmium chloride/human renal proximal tubular cell/protein phosphorylation/connexin 43/siRNA分类
医药卫生引用本文复制引用
刁海鹏,于茂辉,刘庆平,冀晓丽,葛泽河,杜柯贤,吴庆..镉诱导HK-2细胞信号通路的蛋白磷酸化变化及缝隙连接蛋白43的作用[J].环境与职业医学,2016,33(6):562-566,5.基金项目
国家自然科学基金项目(编号:21277033) （编号:21277033）
