环境与职业医学2016，Vol.33Issue(6)：567-570,4.DOI:10.13213/j.cnki.jeom.2016.16176
熊果酸抑制矽肺大鼠转化生长因子β1、白介素1的表达及其作用机制
Inhibitive Effect and Mechanism of Ursolic Acid on TGF-β1 and IL-1 Expression in Silicosis Rat
摘要
Abstract
[Objective] To study possible mechanism of inhibitive effect of ursolic acid on transforming growth factor beta-1 (TGF-β1)and interleukin-1 (IL-1) expressions in silicosis rats.[Methods] Totally 80 Wistar rats were randomly divided into control,model,ursolic acid,and solvent control groups,with 20 rats each.Except the control group,the other groups were induced by intratracheal instillation of silica (SiO2) (250 mg/kg).Then 40 mg/kg ursolic acid,0.6％ (mass fraction) sodium carboxymethyl cellulose (10 mL/kg) solution,and normal saline (10 mL/kg) were given to ursolic acid,solvent control,and control groups respectively for 56consecutive phosphorylated days.ELISA was used to detect serum IL-1 and TGF-β1 in rats.Immunohistochemical and Western blot assays were applied to detect phosphorated protein kinase B (p-AKT1) in lung tissues.[Results] Compared with the control group,the contents of IL-1 and TGF-β1 and the expression of p-AKT1 were significantly higher in the model group and the solvent control group (P ＜ 0.05).However,down-regulations were observed in the ursolic acid group compared with the model group and the solvent control group (P＜0.05).According to the linear regression analysis,the correlation coefficients of the contents of IL-1 and TGF-β1with p-AKT1 at four time points were greater than 0 (P＜0.01).[Conclusions] Ursolic acid could reduce the expression of IL-1 and TGF-β1 and alleviate the development of silicosis probably by inhibiting p-AKT1 activation.关键词
熊果酸/白介素1(IL-1)/转化生长因子β1(TGF-β1)/磷酸化蛋白激酶B/矽肺/大鼠Key words
ursolic acid/interleukin-1/transforming growth factor beta-1/phosphorated protein kinase B/silicosis/rat分类
医药卫生引用本文复制引用
彭海兵,王永恒,曹福源,朱丽艳,刘岩,肖永红..熊果酸抑制矽肺大鼠转化生长因子β1、白介素1的表达及其作用机制[J].环境与职业医学,2016,33(6):567-570,4.基金项目
唐山市科技局科研基金项目(编号:14130262B) （编号:14130262B）
