环境与职业医学2016，Vol.33Issue(6)：571-574,4.DOI:10.13213/j.cnki.jeom.2016.15562
苯暴露对小鼠血液系统和DNA氧化损伤的影响
Effects of Benzene on Blood System and DNA Oxidative Damage in Mice
摘要
Abstract
[Objective] To observe the changes of peripheral hemogram,urinary S-phenylmercapturic acid (SPMA),and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in mice as indicators of blood system and DNA oxidative damage after benzene inhalation.[Methods] A total of 120 Kunming male mice were exposed to 0,375,750,and 1 500mg/m3 of benzene for 2h each day,5 days each week,in static inhalation cabin for three months.In each group ten mice were randomly sacrificed every month.Hemogram and urinary SPMA and 8-OHdG were measured.[Results] With higher benzene exposure dose,the counts of white blood cells (WBC),red blood cells (RBC),and lymphocytes were reduced (P＜0.05),but the levels of SPMA and 8-OHdG in urine were increased (P＜0.05).Higher urinary 8-OHdG level was found in the mice with three months of 1500 mg/m3 benzene inhalation than those with one or two months of exposure (P ＜ 0.05).Urinary SPMA and 8-OHdG were negatively correlated with WBC (rSPMA=-0.718,r8-OHdG=-0.971,P ＜0.01).[Conclusion] Subehronic exposure to benzene might induce blood system and DNA oxidative damage in mice.关键词
苯/静式吸入染毒/血象/苯巯基尿酸/8-羟基脱氧鸟苷Key words
benzene/static inhalation/hemogram/S-phenylmercapturic acid (SPMA)/8-hydroxy-2'-deoxyguanosine(8-OHdG)分类
医药卫生引用本文复制引用
丁婷婷,王茜,郑国颖,刘英莉,袁聚祥,沈福海,蒋守芳,冯福民,金玉兰..苯暴露对小鼠血液系统和DNA氧化损伤的影响[J].环境与职业医学,2016,33(6):571-574,4.基金项目
河北省科技计划项目(编号:13277709D) （编号:13277709D）
