环境与职业医学2016，Vol.33Issue(6)：624-626,3.DOI:10.13213/j.cnki.jeom.2016.15533
固相萃取-超高效液相色谱法测定饮用水中四环素类抗生素
Determination of Tetracyclines in Drinking Water by Ultra High Performance Liquid Chromatography Coupled with Solid Phase Extraction
李勇竞 1郑妹凤1
作者信息
- 1. 芜湖市疾病预防控制中心,安徽 芜湖 241000
- 折叠
摘要
Abstract
[Objective] To build a method to determine tetracyclines in drinking water by ultra high performance liquid chromatography (UPLC) coupled with solid phase extraction.[Methods] Water sample (200mL) was added to Oasis HLB solid phase extraction column at 10-15 mL/min,then eluted by methanol and ethylaeetate mixture (v/v,1 ∶ 9),dried with nitrogen,and dissolved to the mark of 1.0mL using 30％ methanol solution.Then 10 μL final processed sample was injected in UPLC.[Results] The standard curves for the four tetracyclines were linear over the concentration range of 0.05-1.00mg/L,with correlation coefficients all above 0.998.The limits of detection were 0.063-0.250 μg/L.The average recoveries ranged from 77.2％to 136.0％,and the relative standard deviations were in the range of 0.88％ to 5.86％.No selected tetracyclines were detected in 12samples of treated water and tap water.[Conclusion] This method has advantages of simple pre-treatment,rapid UPLC analysis,wide linear range,and low limits of detection,so it can be used to detect tetracyclines in drinking water.关键词
固相萃取/超高效液相色谱/饮用水/四环素类/抗生素Key words
solid phase extraction/ultra high performance liquid chromatography/drinking water/tetracyclines/antibiotics分类
医药卫生引用本文复制引用
李勇竞,郑妹凤..固相萃取-超高效液相色谱法测定饮用水中四环素类抗生素[J].环境与职业医学,2016,33(6):624-626,3.
