水产学报2016，Vol.40Issue(5)：665-672,8.DOI:10.11964/jfc.20150309781
Figla在尼罗罗非鱼卵巢分化和维持过程中的作用
Functional studies of Figla in the ovarian differentiation and maintenance in Nile tilapia (Orechromis niloticus)
摘要
Abstract
Figla (factor in the germ line,alpha),encoding a germ cell-specific transcription factor,is a central regulator of oocyte-specific genes.In order to explore the potential role of Figla in fish gonadal differentiation,tilapia Figla gene was successfully targeted by TALENs (transcription activator-like effector nucleases) in XX individual and confirmed by genomic PCR and subsequent enzyme cleavage and sequencing.Consequently,the morphological and molecular changes in XX gonads were observed by H.E,IHC.Meanwhile,the alterations of E2 and 11-KT were also determined.From screening of different mutant genotypes with bases deletions (4,8,20,etc.),it was revealed that the efficiency of Figla gene targeting by TALEN was about 56.76％.Furthermore,deficiency ofFigla gene in XX fish led to development of abnormal ovaries with severe degeneration or complete loss of oocytes and ectopic proliferations of somatic cells.Amazingly,IHC in Figla-deficient XX gonads revealed that male specific marker gene (Cyp11b2) for 11-KT production in leydig cells was expressed in the cells surrounding oocytes,however,Cyp19a1a was barely detectable which is strongly expressed in follicular cells of normal ovary.Notably,Figla deficiency led to dramatic decrease of E2 serum level along with augmentation in serum 11-KT level.Taken together,our data indicated that Figla might play an essential role in the ovarian differentiation and maintenance in tilapia fish.关键词
尼罗罗非鱼/Figla/卵巢分化和维持/TALENKey words
Orechromis niloticus/Figla/ovarian differentiation and maintenance/TALEN分类
生物科学引用本文复制引用
周林燕,邱永秀..Figla在尼罗罗非鱼卵巢分化和维持过程中的作用[J].水产学报,2016,40(5):665-672,8.基金项目
国家自然科学基金青年基金(31572597) （31572597）
中国高等教育博士科研启动基金(20130182130003) （20130182130003）
西南大学人才引进科研基金(SWU111003) （SWU111003）
中央高校基本科研基金(XDJK2015A004)National Natural Science Foundation of China(31572597) （XDJK2015A004）
Specialized Research Fund for the Doctoral Program of Higher Education of China(20130182130003) （20130182130003）
Research Foundation of Talent Introduction of Southwest University,China (SWU111003) （SWU111003）
Fundamental Research Funds for the Central Universities from Ministry of Education of China (XDJK2015A004) （XDJK2015A004）
