水产学报2016，Vol.40Issue(5)：673-680,8.DOI:10.11964/jfc.20150710000
SOST基因在淇河鲫肌间骨骨化过程中的表达研究
The mRNA and protein expression of gene SOST during ossification process of intermuscular bone in crucian carp (Carassius auratus) in Qihe River
摘要
Abstract
To explore the function of gene SOST in intermuscular bone ossification of Carassius auratus in Qihe River,a modified whole bone staining method was firstly used to investigate ossification development of intermuscular bone.Then,the qRT-PCR,Western Blot and immunohistochemistry methods were employed to detect the mRNA and protein expression of gene SOST in different ossification stages.The results showed that the ligaments were found in myoseptum at 24 dpf (day post fertilization).The earliest epineural bones appeared at 25 dpf,and the epipleural bones were subsequently found at 28 dpf.At 33 dpf,the epineural bones began to bifurcate.Until 45 dpf,all intermuscular bones were fully developed in myoseptum.The gene SOST was expressed in various ossification stages of intermuscular bone and the lowest was at 23 dpf (P＜0.05).The mRNA abundance of gene SOST was increased along with the ossification development and reached a maximum level at 45 dpf (P＜0.05).The relative expression level of SOST protein at 45 dpf was significantly higher than at 23 and 27 dpf (P＜0.05),but had no obvious differences with other ossification stages (P＞0.05).The expression of gene SOST protein showed the similar trend with the mRNA and both of them varied along with the development process of intermuscular bone,which was at the lowest point at 23 dpf and climbed to the highest level when all the intermuscular bones appeared.Thus,we concluded that there were relationships between the gene SOST and ossification development of intermuscular bone,and the gene SOST may play an important role in morphology and ossification of intermuscular bone.关键词
淇河鲫/肌间骨/骨化/硬化蛋白Key words
Carassius auratus/intermuscular bone/ossification/SOST分类
生物科学引用本文复制引用
田雪,王良炎,陈琳,王磊,马晓,胡灿灿,孔祥会,聂国兴,李学军..SOST基因在淇河鲫肌间骨骨化过程中的表达研究[J].水产学报,2016,40(5):673-680,8.基金项目
国家自然科学基金(31402294) （31402294）
河南省教育厅科学技术研究重点项目(14B24005) （14B24005）
河南省重点科技攻关项目(132102110135) （132102110135）
河南省高校科技创新团队支持计划(14IRTSTHN013)National Natural Science Foundation of China (31402294) （14IRTSTHN013）
Educational Commission Program of Henan Province (14B24005) （14B24005）
Key Technologies Research and Development Program of Henan Province (132102110135) （132102110135）
Key Scientific and Technology Project of Henan Province (14IRTSTHN013) （14IRTSTHN013）
