水产学报2016，Vol.40Issue(5)：731-739,9.DOI:10.11964/jfc.20150709955
低盐胁迫下模拟酸雨对裂片石莼光合作用生理特性的影响
Effects of simulated acid rain on the photosynthetic physiological characteristics in Ulva fasciata under salt stress
摘要
Abstract
Ulvafasciata,an extremely rich wild seaweed in natural resources,has a high agricultural and medicinal value.They can also absorb large amounts of nitrogen,phosphorus and other nutrients,and can be used for aquaculture wastewater treatment and the culture environment improvement.With the rapid economic development,industrial pollution gradually increased,the effects of acid rain on terrestrial ecosystems and their harm have received widespread concern,while the effects of acid rain on the photosynthetic physiology of macroalgae in the intertidal zone have barely been studied.This study was to examine the effects of simulated acid rain on the physiological characteristics,including growth rate,chlorophyll content,chlorophyll fluorescence parameters,soluble protein and carbohydrate contents,and antioxidant activities,in hyposaline-grown alga Ulva fasciata.Three salinities (5,10 and 25) and two levels of pH (4.4,8.1) were set in the experiment.The water-pulse amplitude modulated fluorometer,liquid oxygenelectrode,and NBT,coomassie brilliant blue G250 and anthrone colorimetric method were used to determine the combined effects of simulated acid rain and salinity on the macroalgae U.fasciata.(1) The growth of U.fasciata was significantly inhibited by simulated acid rain (pH 4.4),and also affected by salinity of seawater.U.fasciata grown under moderate hyposaline (salinity 10) and normal pH(pH 8.1) showed the highest growth rate.The suppressive effect of simulated acid rain was enhanced by higher salinity.(2) Simulated acid rain (pH 4.4) significantly inhibited the maximum photosynthetic efficiency (Fv/Fm) and the effective photochemical efficiency (Fv'/Fm') of U.fasciata,but the salinity showed no significant effects.The inhibitory effects of simulated acid rain and hyposalinity on the maximum relative electron transfer rate (rETRmax) of U.fasciata have had also been observed.(3) Simulated acid rain (pH 4.4) reduced the dark respiration and photosynthesis oxygen release rate of U.fasciata,but showed no significant difference between salinity treatments.(4) Under normal salinity (25) and pH (pH 8.1) conditions,U.fasciata showed the highest SOD activity.Under relatively high salinity (10,25) treatment,simulated acid rain inhibited the SOD activity of U.fasciata,while under salinity 5 treatment,simulated acid rain increased the SOD activity.(5) Compared with the treatment of pH 8.1,simulated acid rain significantly reduced the soluble protein content of U.fasciata,but the soluble polysaccharide content increased significantly,especially under hyposaline conditions.The effects of simulated acid rain on the physiology of U.fasciata were regulated by seawater salinity.Under the global climate change scenario,the increase of acid rain and salinity might influence the ecosystem of macroalgae grown in the intertidal niches,nearshore ponds and the estuaries.关键词
裂片石莼/低盐/模拟酸雨/光合作用/抗氧化活性Key words
Ulva fasciata/hyposalinity/simulated acid rain/photosynthesis/antioxidant activity分类
农业科技引用本文复制引用
徐晓婷,李亚鹤,王东,徐年军..低盐胁迫下模拟酸雨对裂片石莼光合作用生理特性的影响[J].水产学报,2016,40(5):731-739,9.基金项目
国家自然科学基金(41276122) （41276122）
国家教育部博士点基金博导基金(20123305110002) （20123305110002）
宁波大学科研启动项目(F01259144702) （F01259144702）
宁波大学学科项目(XKL14D2085) （XKL14D2085）
宁波大学“水产”浙江省重中之重学科开放基金(XKZSC1516) （XKZSC1516）
浙江省自然科学基金青年项目(LQ15D060002).National Natural Science Foundation of China (41276122) （LQ15D060002）
National Ministry of Education Doctoral Station Fund and Doctoral Advisor Fund (20123305110002) （20123305110002）
Scientific Research Project of Ningbo University (F01259144702) （F01259144702）
Subject Project of Ningbo University (XKL14D2085) （XKL14D2085）
Ningbo University "Fisheries" Priority Subject Open Fund of Zhejiang Province (XKZSC1516) （XKZSC1516）
Nature science Foundation of Zhejiang Province (LQ15D060002) （LQ15D060002）
