水产学报2016，Vol.40Issue(5)：740-750,11.DOI:10.11964/jfc.20150810032
饲料溶菌酶添加水平对氨氮应激下吉富罗非鱼血清生化指标、抗菌性能和肝脏抗氧化能力的影响
Effects of dietary lysozyme supplementation on serum biochemical indices, antibacterial properties and liver antioxidant capacity of GIFT tilapia (Oreochromis niloticus) under ammonia-nitrogen stress
摘要
Abstract
This study was designed to investigate the effects of dietary lysozyme supplementation on serum biochemical indices,antibacterial properties and liver antioxidant capacity of GIFT tilapia (Oreochromis niloticus) under ammonia-nitrogen stress.Juvenile tilapia with the initial weight of (11.35±0.08) g were fed with six experimental diets (labeled as L0,L18,L36,L54,L72 and L90) which were supplemented with graded lysozyme levels (0,18,36,54,72 and 90 mg/kg) in basal diet for 60 days.After that,a 24 h ammonia-nitrogen stress test with ammonia chloride was conducted to the experimental fish above.The results showed that:① After stress,fish serum biochemical indices presented a significant difference and the fish showed different feedback responses among groups (P＜0.05).The fish in L54 group could reduce damage from ammonia-nitrogen stress through stimulating the immune system and regulating protein metabolism;while mainly through regulating the dynamic variation between high/low density lipoprotein cholesterol,cholesterol and triglyceride in L72 and L90 groups.② Serum antibacterial test showed that there was the maximum resistance ability to Escherichia coli,Staphylococcus aureus,Aeromonas hydrophila in L54 and L72 groups and higher inhibiting ability to Vibrio alginolyticus in L36～L90 groups (P＜0.05),while lysozyme supplementation was helpful for the proliferation of Bacillus subtilis (P＜0.05).③ Liver superoxide dismutase,catalase and glutathione peroxidase activity after ammonia-nitrogen stress were generally enhanced with increased dietary lysozyme level and the highest value was presented in L54 group (P＜0.05);Malonaldehyde content in each lysozyme supplemented group was significantly lower than the control group (P＜0.05).In summary,our results indicate that ingestion of a basal diet supplemented with 54 and 72 mg/kg lysozyme could produce a most positive and effective regulatory response on serum biochemical indices,antibacterial properties and liver antioxidant indices under ammonia-nitrogen stress,so as to improve fish anti-stress ability.关键词
吉富罗非鱼/溶菌酶/氨氮应激/血清生化指标/肝脏抗氧化酶/抗菌活性Key words
GIFT Oreochromis niloticu/lysozyme/ammonia-nitrogen stress/serum biochemical indices/liver antioxidant enzyme/antimicrobial activity分类
水产学引用本文复制引用
王坛,华雪铭,朱伟星,吴钊,孔纯,何亚丁,苏美英..饲料溶菌酶添加水平对氨氮应激下吉富罗非鱼血清生化指标、抗菌性能和肝脏抗氧化能力的影响[J].水产学报,2016,40(5):740-750,11.基金项目
广东省教育部产学研结合项目(2012B091100372) （2012B091100372）
现代农业产业技术体系专项(CARS-49-04B) （CARS-49-04B）
水产动物遗传育种中心上海市协同创新中心(ZF1206)Ministry of Education of Guangdong Province University-industry Cooperation Project (2012B091100372) （ZF1206）
China Agriculture Research System (CARS-49-04B) （CARS-49-04B）
Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206) （ZF1206）
