药学研究2016，Vol.35Issue(7)：382-385,4.DOI:10.13506/j.cnki.jpr.2016.07.004
HPLC - ELSD 法检查抗感颗粒中山银花皂苷类成分
Inspection of saponins from Lonicerae flos in Kanggan Granules by HPLC - ELSD
摘要
Abstract
Objective To establish an HPLC - ELSD method for the inspection of Shanyinhua( Lonicerae flos)in Kanggan Granules. Methods Macranthoidin B and dipsacoside B,which are both characteristic triterpenoid saponins of Lonicerae flos,were used as the detection targets. The method was carried out on a Thermo Hypersil Gold C18 column(4. 6 mm × 250 mm,5 μm)with the mobile phase consisted of water - acetonitrile by gradient elution. The column temperature was 32 ℃,and the flow rate was 1. 0 mL·min - 1 . The drift tube and atomization temperatures of ELSD were set at 90 ℃and 60 ℃,respectively,with the flow rate of N2 at 1. 6 L·min - 1 . Results 53 batches of samples were measured. At least one characteristic triterpenoid saponin of Lonicerae flos was detected in 49 batches of samples,which indicated Lonicerae flos had been used in corresponding batches of Kanggan Granules. Conclusion The established method was highly accurate, specific,and reliable,which can be applied to detect the use of Lonicerae flos in Kanggan Granules.关键词
抗感颗粒/金银花/山银花/灰毡毛忍冬皂苷乙/川续断皂苷乙Key words
Kanggan Granules/Lonicerae japonicae flos/Lonicerae flos/Macranthoidin B/Dipsacoside B分类
药学引用本文复制引用
刘青芝,王立国,崔伟亮,林永强,郭东晓..HPLC - ELSD 法检查抗感颗粒中山银花皂苷类成分[J].药学研究,2016,35(7):382-385,4.基金项目
国家十二五＂重大新药创制＂专项课题-中药质量安全检测和风险控制技术平台（No.2014ZX09304307-002）；山东省自然科学基金项目 （）
