药学研究2016，Vol.35Issue(7)：396-398,407,4.DOI:10.13506/j.cnki.jpr.2016.07.008
五圣伏龙胶囊定性定量方法研究
Study on qualitative and quantitative method for Wushengfulong Capsules
牟卫伟 1刘艳 2孙娟3
作者信息
- 1. 烟台大学药学院，山东 烟台 264005
- 2. 烟台市食品药品检验检测中心，山东 烟台 264670
- 3. 解放军第 406 医院，辽宁 旅顺 116041
- 折叠
摘要
Abstract
Objective To establish a qualitative and quantitative method of Wushengfulong Capsules. Methods Di-oscoreae rhizome were identified by microscope. Paeoniae radix rubra was identified by TLC. HPLC method was used to de-termine the effective content of paeoniflorin in Paeoniae radix rubra,The HPLC method was carried on C18 column using acetonitrile - 0. 1% phosphoric acid solution(14∶86)as mobile phase,and the detection wavelength was 230 nm,the flow rate was 1. 0 mL·min - 1 and the temperature of column was 30 ℃ . Results Dioscoreae rhizome,Paeoniae radix rubra can be identified by TLC. Paeoniflorin showed a good linear relationship in the range of 0. 060 2 ~ 1. 806 μg(r = 0. 999 5),the average recovery was 99. 47%(n = 9). Conclusion This method was simple,reliable,accurate,can be used to control the quality of Wushengfulong Capsules.关键词
五圣伏龙胶囊/赤芍/显微鉴别法/薄层色谱法/高效液相色谱法Key words
Wushengfulong Capsules/Paeoniae radix rubra/Microscopic identification/TLC/HPLC分类
药学引用本文复制引用
牟卫伟,刘艳,孙娟..五圣伏龙胶囊定性定量方法研究[J].药学研究,2016,35(7):396-398,407,4.
