生态环境学报2016，Vol.25Issue(4)：711-714,4.DOI:10.16258/j.cnki.1674-5906.2016.04.023
纳米银污染对蚕豆根尖微核率影响及评价研究
Research on the Influence and Evaluation of Nano-silver Pollution on the Micronucleus Rate of the Root Tip of Vicia Faba
摘要
Abstract
Research on the silver antimicrobial material is very active in recent years, and even promoted the use of nanoparticles to purify water. But how much water nano-silver concentration would cause harm to biological cells has not been reported in studies yet. In this paper, we studied the genetic toxicity of nano-silver in the water by applying the micronucleus technique on the root tip of Vicia faba to provide theoretical basis for evaluating ecological security. By applying different concentrations of nano-silver solution on the root tip of Vicia faba and comparing the micronucleus rate, usingP < 0.01 as a criteria that has statistical significance to determine the significance of the differences of nano-silver concentrations. According to the pollution index criteria standard defined in “National Biotechnology monitoring standards micronucleus technology of root tip of Vicia faba”, we evaluated the degree of pollution of the root tip of Vicia faba in nano-silver solution. The result shows that if the nano-silver solution is 5 µg·mL-1, the micronucleus rate will be 3.33‰, and the pollution index could be 1.11, that is, almost unpolluted, it is conclude that little significant difference can be found between that and the control group; If increase the concentration of nano-silver solution to 50 µg·mL-1, the micronucleus rate will be 4.67‰ with the pollution index of 1.56, which is, lightly polluted. While increasing the content of nano-silver solution, the micronucleus rate and the pollution index of the root tip of Vicia faba gradually increases. When the nano-silver's concentration is 100, 150 µg·mL-1, the micronucleus rate is respectively 6.67‰, 10.67‰ and the pollution index is 2.22 (moderately polluted), 3.56 (heavily polluted), respectively. Multiple comparsion shows that although the difference of micronucleus rate is not significant when the concentration is 50 µg·mL-1 compared to 5 µg·mL-1, the difference is outstanding and statistically significant (P<0.01) if compared to the controlled group. Thus, we can conclude that toxicity can affect cells when the concentration of nano-silver in water reaches 50 µg·mL-1 or higher.关键词
纳米银/蚕豆根尖细胞微核技术/微核率/污染指数/差异显著性Key words
nano-silver/vicia faba/root tip cells’ micronucleus technique/micronucleus rate/pollution index/statistical significance分类
资源环境引用本文复制引用
黄德超,任强,刘蓉,胡淑芬,张重文,张世杰,黄德娟..纳米银污染对蚕豆根尖微核率影响及评价研究[J].生态环境学报,2016,25(4):711-714,4.基金项目
抚州市科技局科技专项项目（抚赣教指[2014]69号）；国家自然科学基金项目 （）
