西北国防医学杂志2016，Vol.37Issue(6)：367-370,4.DOI:10.16021/j.cnki.1007-8622.2016.06.006
高渗盐羟乙基淀粉溶液联合己酮可可碱对重度失血性休克大鼠再灌注后肝脏的影响
Effects of hypertonic saline/hydroxyethyl starch solution combined with pentoxifylline on the livers of rats with severe hemorrhagic shock after reperfusion
摘要
Abstract
Objective:To observe the effects of hypertonic saline/hydroxyethyl starch solution (HSH)combined with pentoxifylline (PTX) on the livers of rats with severe hemorrhagic shock after reperfusion and to reveal the underlying mechanisms.Methods:Seventy-two Sprague-Dawley rats were randomly divided into 6 groups,including control group (group C),shock group (group NR),lactated Ringer's solution-treated group (group LR),LR solution and PTX group (group LR/PTX),HSH-treated group(group HSH),HSH and PTX group (group HSH/PTX group),with 12 rats in each group.The hemorrhagic shock model was made through improved Wigger' s method.The contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in blood plasma,the expression of endothelin-1 (ET-1),nuclear factor-κB (NF-κB),tumor necrosis factor-α (TNF-α),MIP-2 and myeloperoxidase (MPO) activity in liver tissue were detected.Morphological changes of liver were observed under the light and electron microscopes.Results:Compared with group LR or HSH,the content of AST and ALT in blood plasma,expression of NF-κB,ET-1,TNF-α,MIP-2 and MPO activity in liver tissue were significantly decreased in group LR/PTX and HSH/PTX (P ＜ 0.05).Liver injury was obviously less especially in group HSH/PTX.Conclusion:Liver injury can be well protected by HSH/PTX probably through reducing the production of ET-1,TNF-α and MIP-2,suppressing the activation of NF-κB,and reducing the activity of MPO in rats with severe hemorrhagic shock after perfusion.关键词
失血性休克/肝损伤/己酮可可碱/高渗盐羟乙基淀粉溶液/炎症反应Key words
ehemorrhagic shock/liver injury/pentoxifylline/hypertonic saline/hydroxyethyl starch solution/inflammation respons分类
基础医学引用本文复制引用
耿智隆,陆化梅,曹虹,向许进,杨木强,赵峰..高渗盐羟乙基淀粉溶液联合己酮可可碱对重度失血性休克大鼠再灌注后肝脏的影响[J].西北国防医学杂志,2016,37(6):367-370,4.基金项目
全军“十一五”面上基金资助项目(06MA083) （06MA083）
