信阳师范学院学报（自然科学版）2016，Vol.29Issue(3)：431-434,4.DOI:10.3969/j.issn.1003-0972.2016.03.028
ASE-UPLC 法同时测定杜仲制剂中3种成分
Simultaneous Determination of Three Components in Preparations Containing Eucommia Ulmoides by a Method of ASE-UPLC
摘要
Abstract
An ASE-UPLC method for simultaneous determination of three components including geniposidic acid,chlorogenic acid and pinoresinol diglucoside in the different preparations containing Eucommia ulmoides was established.The ASE extraction was conducted with 70% MeOH-H 2 O as solvent at 40 ℃ and 103.4 MPa, and UPLC analysis was performed on an Acquity UPLC BEH C18 column (100 nm×2.1 mm,1.7 μm)with acetonitrile (A)-0.1% formic acid (B)as mobile phases for gradient elution (0 ~1 5 min,70% B~90% B)at the flow rate of 0.1 5 mL/min.The detection wavelength was 237 nm and the column temperature was 20 ℃. The results showed that three components were separated completely and showed good linearity,precision and repeatability (RSD <3.0% respectively).Recoveries were between 95.84% ~ 102.69% (RSD < 3.0%).The data suggested the established method was rapid,accurate,good reproducible and could be applied to simultane-ously determine main components in the preparations containing Eucommia ulmoides .关键词
杜仲制剂/ASE-UPLC/京尼平苷酸/绿原酸/松脂醇二葡萄糖苷Key words
Eucommia ulmoides/ASE-UPLC/geniposidic acid/chlorogenic acid/pinoresinol diglucoside分类
医药卫生引用本文复制引用
李欣,袁培耘,何亚平,杨庆雄..ASE-UPLC 法同时测定杜仲制剂中3种成分[J].信阳师范学院学报（自然科学版）,2016,29(3):431-434,4.基金项目
国家自然科学基金项目(31160076) （31160076）
