诊断学理论与实践2016，Vol.15Issue(3)：263-267,5.DOI:10.16150/j.1671-2870.2016.03.015
单形拟杆菌减轻组织脂质沉积及内质网应激改善小鼠葡萄糖代谢
Bacteroides uniformis supplementation improves glucose homeostasis by alleviating lipid accumulation and endo-plasmic reticulum stress in liver/muscle in chow-diet mice
摘要
Abstract
Objective: To investigate the metabolic benefits of Bacteroides uniformis, a potential probiotic, in healthy subjects, and to provide evidence for benefit from probiotic supplementation. Methods: Chow-diet fed C57BL6/J mice were treated with B.uniformis 2×108 colony-forming units (CFUs)/200μL by daily gavage for five weeks, while their coun-terparts were treated with sterile PBS. Expressions of relative genes involved in lipid biosynthesis, fatty acid import and endoplasmic reticulum stress (ER stress) in insulin-responsive tissues (liver and muscle) were determined by real-time PCR. Results:B.uniformis supplementation significantly improved glucose tolerance in chow-diet fed mice (P<0.05) with-out affecting body weight gain and fat mass. The relative mRNA expression of lipid biosynthesis gene SREBP1C, fatty acid transport regulator CD36 and ER stress-related genes were decreased after B.uniformis supplementation (P<0.05). Conclusions: B.uniformis supplementation ameliorates ectopic lipid accumulation and ER stress in liver and muscle, thereby improving glucose homeostasis of chow-diet fed mice. These results provide the possible mechanism of B.uni-formis′s metabolic benefits, which provides new evidence for the supplementation of probiotic to healthy subjects and sug-gests a new strategy to prevent the development of metabolic disorders at earlier stage.关键词
Bacteroidesuniformis/葡萄糖耐量/异位脂质沉积/内质网应激Key words
Bacteroidesuniformis/Glucose tolerance/Ectopic lipid accumulation/Endoplasmic reticulum stress分类
临床医学引用本文复制引用
赵少倩,刘文,石娟,王计秋,刘瑞欣,洪洁..单形拟杆菌减轻组织脂质沉积及内质网应激改善小鼠葡萄糖代谢[J].诊断学理论与实践,2016,15(3):263-267,5.基金项目
上海市卫生系统优秀学科带头人培养计划 （）
