中国动脉硬化杂志2016，Vol.24Issue(7)：657-662,6.
降钙素基因相关肽对c-kit+心脏干细胞生存活力的影响
The Influence of CGRP on the Cell Activity of c-kit+ Cardiac Stem Cells
摘要
Abstract
Aim To investigate the influence of calcitonin gene-related peptide (CGRP) on the survival activity of c-kitpos cardiac stem cells(c-kit+ CSC) in hypoxia,and research its potential mechanism.Methods c-kit+ CSC was obtained by enzyme digestion and immune magnetic bead separation.Then serum-free hypoxia model of c-kit+ CSC was established in vitro.The models were randomly divided into hypoxia group,CGRP+hypoxia group,CGRP+CGRP8-37+hypoxia group and control group(no hypoxia).The activity of cells was detected by cell counting kit (CCK-8 kit),the apoptosis of ceils were detected by flow cytometry(FCM) and mitochondrial membrane potential.Results Under hypoxia condition,compared with the control group,the activity of c-kit+ CSC was significantly decreased in the hypoxia group(P＜0.05),but compared with the hypoxia group,the activity of c-kit+ CSC was significantly increased in CGRP+hypoxia group at different time points of CGRP effect on hypoxia cells(P＜0.05),especially in 30 min and 60 min; When inhibiting the effect of CGRP on hypoxia,compared with CGRP+hypoxia group,the activity of c-kit+ CSC was significantly decreased in the CGRP+CGRP8-37 +hypoxia group at 30 min and 60 min after hypoxia(P＜0.05).In terms of apoptosis,compared with the control group,the early apoptosis rate was the highest in hypoxia group(P＜0.05) ; After CGRP treating c-kit+ CSC in hypoxia,we could find that CGRP+hypoxia group had a lower early apoptosis rate(P＜0.05),when inhibiting the effect of CGRP on hypoxia,the early apoptosis rate was the highest in CGRP+CGRP8-37 +hypoxia group,but there was no significant difference in early apoptosis rate between hypoxia group and CGRP+CGRP8-37+hypoxia group(P＞0.05).Mitochondrial membrane potential showed,compared with the hypoxia group,red fluorescence and green fluorescence ratio increased in CGRP+hypoxia group(P＜0.05),when inhibiting the effect of C GRP on hypoxia,compared with the CGRP+hypoxia,the red fluorescence and green fluorescence ratio decreased in CGRP+CGRP8-37+hypoxia group(P＜0.05).Conclusion CGRP can promote the proliferation and survival of c-kit+CSC under hypoxia,and inhibit the early apoptosis of the cell.关键词
降钙素基因相关肽/c-kit+心脏干细胞/细胞存活率/细胞凋亡Key words
Calcitonin Gene Related Peptide/c-kit+ Cardiac Stem Cells/Cell Activity/Cell Apoptosis分类
医药卫生引用本文复制引用
龙仙萍,郑小宇,邓文文,石蓓..降钙素基因相关肽对c-kit+心脏干细胞生存活力的影响[J].中国动脉硬化杂志,2016,24(7):657-662,6.基金项目
国家自然科学基金(81360021) （81360021）
贵州省国际合作项目[黔科合外G字(2013)7037号] （2013）
