中国动脉硬化杂志2016，Vol.24Issue(7)：663-667,5.
阿托伐他汀对脂多糖诱导的THP-1巨噬细胞炎症因子分泌的影响及机制
The Effect and Mechanism of Atorvastatin on the THP-1 Macrophage Proinflammatory Cytokines Secretion Induced by Lipopolysaccharide
摘要
Abstract
Aim To investigate the effect and potential mechanism of atorvastatin on the THP-1 macrophage proinflammatory cytokines release induced by lipopolysaccharide (LPS).Methods THP-1 cells were treated with phorbol 12-myristate 13-acetate (100 nmol/L) for 24 h to differentiate into macrophages.The medium was then replaced with serum-free medium containing LPS and (or) atorvastatin.The secretion of interleukin-1β (IL-1 β) and interleukin18 (IL-18) were quantitated using enzyme-linked immunosorbent assay analysis.The mRNA level of nucleotide-binding oligomerization domain-like receptor protein 1 (NLRP1) inflammasome was measured by real-time PCR.Western blot was employed to analyze the protein expression of NLRP1 inflammasome.Results Atorvastatin inhibited IL-1β and IL-18 secretion induced by LPS in THP-1 macrophages in a dose-and time-dependent manner.Atorvastatin decreased the mRNA and protein expression of NLRP1 inflammasome in THP-1 macrophages.Conclusion Atorvastatin reduces proinflammatory cytokines release from macrophages,and the mechanism might be related to the inhibition of NLRP1 inflammasome expression.关键词
阿托伐他汀/抗炎效应/核苷酸结合寡聚化结构域样受体蛋白1炎性体/动脉粥样硬化Key words
Atorvastatin/Anti-inflammatory Effect/Nucleotide-binding Oligomerization Domain-like Receptor Protein 1 Inflammasome/Atherosclerosis分类
医药卫生引用本文复制引用
喻思扬,曾高峰,刘洋,徐健强,曾梦雅,唐业华,曾志英,石小桥,陈莹..阿托伐他汀对脂多糖诱导的THP-1巨噬细胞炎症因子分泌的影响及机制[J].中国动脉硬化杂志,2016,24(7):663-667,5.基金项目
国家自然科学基金资助项目(81200218) （81200218）
湖南省自然科学基金课题(14JJ5016) （14JJ5016）
