中国动脉硬化杂志2016，Vol.24Issue(7)：668-672,5.
内质网应激介导了高糖引起的血管平滑肌细胞钙化
Endoplasmic Reticulum Stress Mediates High Glucose-induced Calcification of Vascular Smooth Muscular Cells
摘要
Abstract
Aim To investigate whether high blood glucose-induced vascular calcification in diabetes mellitus is caused by the endoplasmic reticulum(ER) response and subsequent apoptosis.Methods We examined the effects of high glucose on the ER stress response and calcification of vascular smoothmuscle cells (VSMC).Alkaline phosphatase (ALP) activity,calcium content and osteogenic markers expression was measured by colorimetric assay,o-cresolphthalein method and Western blot analysis.Results High glucose induced osteoblastic differentiation and ER stress of VSMC,as evidenced by ALP activity,calcium content and osteogenic markers expression increase.4-PBA treatment could inhibit ER stress and apoptosis of VSMC,therefore,high glucose-elicited VSMC calcification was retarded.Conclusion High glucose can activate ER stress and osteoblastic differentiation in VSMC,ER stress and apoptosis might mediate VSMC calcification induced by high glucose.关键词
高糖/内质网应激/血管平滑肌细胞/血管钙化Key words
High Glucose/Endoplasmic Reticulum Stress/Vascular Smooth Muscle Cell/Vascular Calcification分类
医药卫生引用本文复制引用
郭润民,刘畅,吴斌,李兴岳,姜佳美,游琼,胡洁芬,朱强,吴铿..内质网应激介导了高糖引起的血管平滑肌细胞钙化[J].中国动脉硬化杂志,2016,24(7):668-672,5.基金项目
广东省自然科学基金博士启动项目(2015A030310359) （2015A030310359）
湛江市财政资金科技专项竞争性分配项目(2014A01033) （2014A01033）
广东省重大科技专项(2012A080202020) （2012A080202020）
湛江市第二批科技计划项目(2012C0303) （2012C0303）
