中国临床药理学与治疗学2016，Vol.21Issue(5)：481-485,5.
内皮衍生超极化因子/硫化氢对小鼠神经细胞的超极化作用
Hyperpolarization of endothelium-derived hyperpolarizing factor/hydrogen sulfide on nerve cell membrane potential in mice
摘要
Abstract
AIM:The present study examined the hyperpolarizing effects of endothelium derived hyperpolarizing factor (EDHF) / hydrogen sulphide (H2S) on the membrane potential of the nerve cell in mice.METHODS:In presence of 3 × 10-5 mol/L N-nitro-L-arginine-methyl-ester (L-NAME,an inhibitor of nitric oxide synthase) plus 10-5mol/L indomethacin (Indo,an inhibitor of PGI2 synthsis),to evaluate the hyperpolarizing effect of acetylcholine (ACh,10-5.5-10-8 mol/L) on nerve cell in CSE+/+ and CSE knockout (CSE-/-) mice and NarHS (10-5-10-2.5 mol/L) on the nerve cell in CSE+/+ mice.RESULTS:ACh (10-8-10-5.5 mol/L) induced concentration-dependent hyperpolarization nerve cell in CSE+/+ mice while Significantly reduced by remove MCA/Endo or PSS replace.The non NO/PGI2 responses induced by ACh were abolished by the co-application of 10-7 mol/L charybdotoxin (ChTX) and 5 × 10-6 mol/L apamin (Apa)which blocked intermediate-and small-conductane calcium-activated potassium (IKca and SKca) channels.The endogenous H2S synthase inhibitor 10-4 mol/L DL-propargylglycine (PPG) or CSE-/-were also abolished the effect.The H2 S donor NarHS (10-5-10-2.5 mol/L) elicited concentration-dependent hyperpolarization nerve cell in CSE +/+ mice and was inhibited by 10-7 mol/L Iberiotoxin (IBTX) which blocked large-conductane Kca (BKca) channels.CONCLUSION:H2S act as EDHF causes nerve cell hyperpolarization.关键词
内皮衍生超极化因子/硫化氢/大脑中动脉/钙激活钾离子通道/神经细胞Key words
endothelium derived hyperpolarizing factor/hydrogen sulphide/middle cerebral artery/calcium-activated potassium channels/nerve cell分类
医药卫生引用本文复制引用
陈方林,胡金兰,陈志武..内皮衍生超极化因子/硫化氢对小鼠神经细胞的超极化作用[J].中国临床药理学与治疗学,2016,21(5):481-485,5.基金项目
国家自然科学基金(81173596,81374002) （81173596,81374002）
